Beutelspacher S C, Serbecic N, Tamaddon F, Mehrabi M, Völcker H E
Universitäts-Augenklinik, Universität Heidelberg.
Ophthalmologe. 2003 Nov;100(11):955-9. doi: 10.1007/s00347-003-0818-y.
Recent data indicate that lipid peroxidation is implicated in the pathogenesis of giant cell arteritis with a close anatomic relationship between reactive oxygen species and oxidatively injured vascular tissue.
Immunohistochemistry utilizing anti-ox-LDL was performed on paraffin sections of isolated temporal arteries obtained from patients (n=23) suspected of having temporal arteritis. Enrichment as well as staining intensity of ox-LDL in vascular tissue was analysed by digital image planimetry.
Temporal arteries with biopsy proven temporal arteritis (n=11) presented with significantly higher enrichment of ox-LDL in the intima (16.9+/-4.2% vs. 11.25+/-2.3%; p<0.01) and mean (9.6+/-2.4% vs. 6.75+/-1.8%; p<0.01) as compared to healthy controls. Comparable results for the staining intensity were found in the intimal (2.8+/-0.5 eU vs. 1.7+/-0.4 eU; p<0.01) and medial layer (1.55+/-0.5 eU vs. 1.04+/-0.6 eU; p<0.01) of diseased patients compared to controls.
Accumulation of ox-LDL in the intimal layer, especially at the intima-media-border, was closely related to disruption of the elastica interna and adjacent vascular tissue, presumably contributing to the underlying process of intimal hyperplasia through unimpeded migration of smooth muscle and accumulation inflammatory cells.
近期数据表明，脂质过氧化与巨细胞动脉炎的发病机制有关，活性氧与氧化损伤的血管组织之间存在密切的解剖学关系。
对从疑似颞动脉炎患者（n = 23）获取的孤立颞动脉石蜡切片进行抗氧化低密度脂蛋白免疫组织化学检测。通过数字图像平面测量法分析血管组织中氧化低密度脂蛋白的富集情况以及染色强度。
经活检证实为颞动脉炎的颞动脉（n = 11），与健康对照相比，内膜中氧化低密度脂蛋白的富集显著更高（分别为16.9±4.2% 对11.25±2.3%；p < 0.01），平均水平也更高（分别为9.6±2.4% 对6.75±1.8%；p < 0.01）。与对照相比，患病患者内膜层（分别为2.8±0.5 eU对1.7±0.4 eU；p < 0.01）和中层（分别为1.55±0.5 eU对1.04±0.6 eU；p < 0.01）的染色强度也有类似结果。
氧化低密度脂蛋白在内膜层尤其是内膜 - 中膜边界处的积累与内弹性膜及相邻血管组织的破坏密切相关，可能通过平滑肌的无阻碍迁移和炎症细胞的积累促进内膜增生的潜在过程。
