Wang A
Institute of Microbiology, Academia Sinica, Beijing.
Yi Chuan Xue Bao. 1992;19(4):362-8.
The first independent vitamin B2 auxotrophs of Salmonella typhimurium were obtained by using selective medium containing extraordinarily high concentration of B2 (300 micrograms/ml) after mutagenesis. Transduction analysis showed that 21 B2 auxotrophs could be divided into four groups, which were unlinked to each other. It means that at least 4 structural genes were involved in B2 biosynthetic pathway. Preliminary genetic mapping indicated that 2 genes located in 7'-22' and other 2 located in 22'-34' and 61.5'-69' region on the genetic map of S. typhimurium respectively.
鼠伤寒沙门氏菌的首个独立维生素B2营养缺陷型菌株是通过在诱变后使用含有极高浓度B2（300微克/毫升）的选择培养基获得的。转导分析表明，21个B2营养缺陷型菌株可分为四组，它们彼此不连锁。这意味着至少有4个结构基因参与了B2生物合成途径。初步遗传图谱显示，2个基因分别位于鼠伤寒沙门氏菌遗传图谱上7'-22'区域，另外2个位于22'-34'区域和61.5'-69'区域。
