Voss G, Stahl-Hennig C, Petry H, Coulibaly C, Nick S, Fuchs D, Wachter H, Lüke W, Hunsmann G
Deutsches Primatenzentrum, Abteilung Virologie und Immunologie, Göttingen, Germany.
AIDS Res Hum Retroviruses. 1992 Aug;8(8):1397-400. doi: 10.1089/aid.1992.8.1397.
Two vaccine trials were conducted with low- and high-dose purified Twen-ether-treated SIVmac adsorbed onto aluminum hydroxide using rhesus macaques. In the first experiment 7 macaques were immunized with a total amount of 560 micrograms protein and 3 animals served as controls. After the immunization period the vaccinees exhibited ELISA titers up to 1:1280 and 5 immunized animals showed an antigen-specific proliferative response. After the virus challenge the 3 control animals and 3 vaccinees became infected. Four of the infected animals developed a cytotoxic T-cell response beginning 8 weeks postchallenge. The 4 protected animals were rechallenged 16 weeks later and all became infected. For the high-dose experiment 5 immunized animals receiving 2 mg of antigen and 2 control animals were used. The ELISA titers of the vaccinees reached 1:20480 and 4 animals exhibited an antigen-specific proliferative response. In response to virus challenge the 2 control and 1 immunized animal became infected. From these data it can be concluded that the high-dose immunization scheme elicited higher antibody titers and increased the fraction of protected animals.
使用恒河猴对吸附于氢氧化铝上的低剂量和高剂量纯化双醚处理的SIVmac进行了两项疫苗试验。在第一个实验中，7只猕猴用总量为560微克蛋白质进行免疫，3只动物作为对照。免疫期后，疫苗接种者的ELISA滴度高达1:1280，5只免疫动物表现出抗原特异性增殖反应。病毒攻击后，3只对照动物和3只疫苗接种者被感染。4只受感染动物在攻击后8周开始产生细胞毒性T细胞反应。4只受保护的动物在16周后再次受到攻击，全部被感染。在高剂量实验中，使用了5只接受2毫克抗原的免疫动物和2只对照动物。疫苗接种者的ELISA滴度达到1:20480，4只动物表现出抗原特异性增殖反应。针对病毒攻击，2只对照动物和1只免疫动物被感染。从这些数据可以得出结论，高剂量免疫方案引发了更高的抗体滴度，并增加了受保护动物的比例。
