重组人促红细胞生成素在体内诱导套叠式微血管生长。
Recombinant human erythropoietin induces intussusceptive microvascular growth in vivo.
作者信息
Crivellato E, Nico B, Vacca A, Djonov V, Presta M, Ribatti D
机构信息
Department of Medical and Morphological Researches, Anatomy Section, University of Udine Medical School, Udine, Italy.
出版信息
Leukemia. 2004 Feb;18(2):331-6. doi: 10.1038/sj.leu.2403246.
The role of erythropoietin (Epo) in angiogenesis has not been completely clarified. Epo induces endothelial cell proliferation and migration and stimulates angiogenesis on rat aortic rings in vitro and in vivo in the chick embryo chorioallantoic membrane (CAM) assay. The aim of the present study was to evaluate the ultrastructural aspects of angiogenesis in the CAM vasculature after recombinant human Epo (rHuEpo) exposure. The results demonstrated that after rHuEpo stimulation, the generation of new blood vessels occurred more frequently following an intussusceptive microvascular growth (IMG) mechanism. We have performed our experiments between days 8 and 12 of incubation, that is, when in the normal condition the capillary network expands mainly by IMG, and because it is generally accepted that implants made from days 8 to 10 are strongly angiogenic. This response is peculiar of rHuEpo, because it is abolished when an Epo-blocking antibody was coadministered with Epo.
促红细胞生成素（Epo）在血管生成中的作用尚未完全阐明。Epo可诱导内皮细胞增殖和迁移，并在体外对大鼠主动脉环以及在鸡胚绒毛尿囊膜（CAM）试验中的体内实验中刺激血管生成。本研究的目的是评估重组人促红细胞生成素（rHuEpo）暴露后CAM脉管系统中血管生成的超微结构特征。结果表明，rHuEpo刺激后，通过套入式微血管生长（IMG）机制形成新血管的情况更为频繁。我们在孵化的第8天至第12天进行了实验，也就是说，在正常情况下，毛细血管网络主要通过IMG扩展，并且通常认为在第8天至第10天植入的组织具有很强的血管生成能力。这种反应是rHuEpo所特有的，因为当Epo阻断抗体与Epo共同给药时，这种反应就会消失。
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