Kirschberg Oliver, Schüttler Christian, Repp Reinald, Schaefer Stephan
Institut für Medizinische Virologie, Justus-Liebig-Universität, Giessen, Germany
J Clin Virol. 2004 Jan;29(1):39-43. doi: 10.1016/s1386-6532(03)00084-2.
Eight genotypes (A-H) of hepatitis B virus (HBV) are known with variations in nucleotide sequences greater than 8%. Several recent publications found that the clinical course and outcome of antiviral therapy depended on the genotype of the infecting HBV strain. Large epidemiological studies will require the availability of a system which is rapid, reliable and can be performed on a large number of samples. We have developed a multiplex-PCR assay which uses genotype-specific primer pairs for HBV genotypes A-F. These primer pairs specifically amplified HBV DNA of the respective genotype, either in single or in multiplex-PCR. Sensitivity of the assay was in the range of 10(4) genome equivalents.
已知乙型肝炎病毒（HBV）有八种基因型（A - H），其核苷酸序列差异大于8%。最近的几篇出版物发现，抗病毒治疗的临床过程和结果取决于感染的HBV毒株的基因型。大规模的流行病学研究将需要一个快速、可靠且能对大量样本进行检测的系统。我们开发了一种多重PCR检测方法，该方法使用针对HBV A - F基因型的基因型特异性引物对。这些引物对在单重或多重PCR中均可特异性扩增相应基因型的HBV DNA。该检测方法的灵敏度在10⁴个基因组当量范围内。
