Suzuki Masao, Zhou Hongning, Hei Tom K, Tsuruoka Chizuru, Fujitaka Kazunobu
ISRL, NIRS.
Biol Sci Space. 2003 Oct;17(3):251-2.
We report here a bystander effect in chromosomal damage using He-ion microbeam. Human-hamster hybrid cells were irradiated with a precision He-ion microbeam generated by the Columbia microbeam system. When 20% of the cells were exposed to single He ion, the incidence of cells with chromatid-type breaks detected with the PCC technique was covered wide range from 0 to 6 breaks per cell. In contrast, the distribution showed a mixed two-peak pattern, such as non-exposed and all-cell exposed patterns, under the condition of assuming no bystander effect by treating with an effective inhibitor of cell-cell communication. These findings provide clear evidence that single He-ion irradiated cells can induce bystander chromosomal alterations in neighboring cells not directly hit by He ion.
我们在此报告使用氦离子微束在染色体损伤中观察到的旁观者效应。用人-仓鼠杂交细胞接受由哥伦比亚微束系统产生的精确氦离子微束照射。当20%的细胞暴露于单个氦离子时，用早熟染色体凝集（PCC）技术检测到的具有染色单体型断裂的细胞发生率覆盖了每个细胞0至6个断裂的广泛范围。相比之下，在通过用细胞间通讯的有效抑制剂处理假设无旁观者效应的条件下，分布呈现出混合双峰模式，如未暴露和全细胞暴露模式。这些发现提供了明确的证据，即单个氦离子照射的细胞可诱导未被氦离子直接击中的邻近细胞发生旁观者染色体改变。
