Kaleczyc Jerzy, Sienkiewicz Waldemar, Klimczuk Magdalena, Czaja Krzysztof, Lakomy Mirosław
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
Folia Histochem Cytobiol. 2003;41(4):201-11.
The present study was designed to investigate and to compare the chemical coding of nerve fibres supplying major populations of neurons in the caudal mesenteric (CaMG) and anterior pelvic (APG) ganglion in juvenile male pigs (n=5) using double-labelling immunofluorescence. The co-existence patterns of some biologically active substances including tyrosine hydroxylase (TH) and vesicular acetylcholine transporter (VAChT) as well as vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin gene-related peptide (CGRP), Leu5-enkephalin (LENK) and serotonin (5-HT) were analysed under a confocal laser scanning microscope. Profound differences in the neurochemical features of the nerve terminals between the ganglia were observed. Moreover, there were also distinct differences in the chemical coding of nerve fibres associated with the particular populations and subpopulations of neurons within the ganglia. In both ganglia, nearly all adrenergic and cholinergic neurons were supplied with VAChT-positive nerve fibres (putative preganglionic fibres). However, in the CaMG, they were more numerous and, in contrast to the APG, many of them also stained for VIP. In the APG, a great number of nerve terminals expressed immunoreactivity to SP and CGRP (putative collaterals of sensory neurons). Interestingly, they densely supplied almost exclusively adrenergic neurons. SP-positive nerve fibres were moderate in number in the CaMG, but, in addition to VAChT-IR nerve terminals, the most numerous populations of nerve fibres in this ganglion were those expressing highly colocalized immunoreactivities to CGRP and LENK, and those which stained for 5-HT (putative processes of enteric neurons). However, these fibres supplied almost exclusively larger, intensely stained for TH and clustered adrenergic neurons. This diversity of the nerve terminals reflects the complexity of nerve circuits involved in the innervation of structures supplied by neurons in the porcine CaMG and APG. It also demonstrates the importance of nerve inputs for the proper function of autonomic neurons and thus their target tissues.
本研究旨在利用双标免疫荧光法，对幼年雄性猪（n = 5）肠系膜尾侧（CaMG）和盆前（APG）神经节中主要神经元群的神经纤维化学编码进行研究和比较。在共聚焦激光扫描显微镜下分析了一些生物活性物质的共存模式，包括酪氨酸羟化酶（TH）、囊泡乙酰胆碱转运体（VAChT）以及血管活性肠肽（VIP）、P物质（SP）、降钙素基因相关肽（CGRP）、亮氨酸脑啡肽（LENK）和5-羟色胺（5-HT）。观察到神经节之间神经末梢的神经化学特征存在显著差异。此外，神经节内与特定神经元群和亚群相关的神经纤维化学编码也存在明显差异。在两个神经节中，几乎所有的肾上腺素能和胆碱能神经元都有VAChT阳性神经纤维（假定的节前纤维）供应。然而，在CaMG中，它们数量更多，与APG不同的是，其中许多还对VIP染色。在APG中，大量神经末梢对SP和CGRP表现出免疫反应性（感觉神经元的假定侧支）。有趣的是，它们几乎只密集地供应肾上腺素能神经元。SP阳性神经纤维在CaMG中的数量适中，但除了VAChT免疫反应性神经末梢外，该神经节中数量最多的神经纤维群体是那些对CGRP和LENK表现出高度共定位免疫反应性的群体，以及那些对5-HT染色的群体（肠神经元的假定突起）。然而，这些纤维几乎只供应较大的、对TH染色强烈且聚集的肾上腺素能神经元。神经末梢的这种多样性反映了猪CaMG和APG中神经元所支配结构的神经回路的复杂性。它还证明了神经输入对自主神经元及其靶组织正常功能的重要性。
