[采用M13引物PCR指纹图谱法检测白色念珠菌对氟康唑的耐药性]
[Fluconazole resistance in Candida albicans assayed by PCR fingerprinting with M13 prime].
作者信息
Wang Ying-Bin, Wang Hong, Guo Hui-Yu, Zhao Yong-Zhong, Luo Shen-Qiu
机构信息
Department of Cell Biology, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2003 Dec;23(12):1303-6.
OBJECTIVE
To understand the molecular and genetic mechanism underlying fluconazole resistance in Candida albicans by PCR fingerprinting with M13 primer.
METHODS
Paper disc diffusion method was employed for assay of fluconazole resistance in 41 clinical isolates of Candida albicans, followed by PCR fingerprinting with M13 primer to study the gel patterns with cluster analysis using neighbor joining (NJ) method performed with RAPD200 software.
RESULTS
Of the 41 clinical isolates, 11 strains (26.8%) were fluconazole-sensitive, 8 (19.5%) fluconazole-dependent and 22 (53.7%) fluconazole-resistance. Two to twelve bands could be observed among these strains, and the gel patterns revealed by cluster analysis were associated with the reactions of the strains against fluconazole and the location of infection.
CONCLUSION
There is high prevalence of fluconazole resistance in clinical Candida albicans isolates, and PCR fingerprinting with M13 primer is convenient for assay of fluconazole resistance and molecular epidemiological study of Candida albicans.
目的
通过M13引物PCR指纹图谱法了解白色念珠菌对氟康唑耐药的分子和遗传机制。
方法
采用纸片扩散法检测41株临床分离的白色念珠菌对氟康唑的耐药性，随后用M13引物进行PCR指纹图谱分析，使用RAPD200软件通过邻接法（NJ）进行聚类分析研究凝胶图谱。
结果
41株临床分离株中，11株（26.8%）对氟康唑敏感，8株（19.5%）为氟康唑依赖型，22株（53.7%）对氟康唑耐药。这些菌株中可观察到2至12条带，聚类分析显示的凝胶图谱与菌株对氟康唑的反应及感染部位有关。
结论
临床分离的白色念珠菌中氟康唑耐药的发生率很高，M13引物PCR指纹图谱法便于检测白色念珠菌对氟康唑的耐药性及进行分子流行病学研究。
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