Wood R I, Brabec R K, Swann J M, Newman S W
Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor 48109-0616.
Brain Res. 1992 Nov 20;596(1-2):89-98. doi: 10.1016/0006-8993(92)91536-n.
The medial preoptic area (MPOA), bed nucleus of the stria terminalis (BNST), and medial amygdaloid nucleus (Me) are essential for male sexual behavior in the Syrian hamster. These nuclei received chemosensory stimuli and gonadal steroid signals, both of which are required for mating behavior. The objective of this study was to compare the distribution of androgen- and estrogen-concentrating neurons in MPOA, BNST, and Me in the adult male hamster using steroid autoradiography for estradiol (E2), testosterone (T) and dihydrotestosterone (DHT). Adult males (n = 4 per group) received two i.p. injections of tritiated steroid 4-7 days after castration. Six-microns frozen sections through the brain were mounted onto emulsion-coated slides, and exposed for 11-16 months. In MPOA, BNST, and Me, neurons were more abundant and heavily labelled after [3H]E2 treatment than after either [3H]T or [3H]DHT. Tritiated estradiol- and DHT-labeled cells were found throughout the rostrocaudal extent of Me, with a high concentration in posterodorsal Me. Tritiated testosterone treatment labelled cells largely within posterodorsal Me. In MPOA, the majority of E2-, T-, and DHT-labelled neurons were in the medial preoptic nucleus (MPN) and the preoptic continuation of the posteromedial bed nucleus of the stria terminalis (BNSTpm). Few T-labelled cells were present outside these subdivisions. In the BNST, E2- and DHT-labelled neurons were present in all subdivisions, whereas T labelling was confined to the antero- and posteromedial subdivisions of BNST. These results suggest that the distribution of androgen- and estrogen receptor-containing neurons overlap considerably in nuclei which transmit chemosensory signals in the control of mating behavior.
内侧视前区（MPOA）、终纹床核（BNST）和内侧杏仁核（Me）对叙利亚仓鼠的雄性性行为至关重要。这些核团接收化学感觉刺激和性腺类固醇信号，这两者都是交配行为所必需的。本研究的目的是使用雌二醇（E2）、睾酮（T）和二氢睾酮（DHT）的类固醇放射自显影术，比较成年雄性仓鼠MPOA、BNST和Me中雄激素和雌激素浓缩神经元的分布。成年雄性仓鼠（每组n = 4）在去势后4 - 7天接受两次腹腔注射氚标记的类固醇。将穿过大脑的6微米冰冻切片安装在涂有乳剂的载玻片上，并曝光11 - 16个月。在MPOA、BNST和Me中，[3H]E2处理后的神经元比[3H]T或[3H]DHT处理后的神经元更丰富且标记更重。在Me的整个前后范围内都发现了氚标记的雌二醇和DHT标记的细胞，在背侧后部Me中浓度较高。氚标记的睾酮处理标记的细胞主要在背侧后部Me内。在MPOA中，大多数E2、T和DHT标记的神经元位于内侧视前核（MPN）和终纹床核后内侧部（BNSTpm）的视前延续部分。在这些亚区之外几乎没有T标记的细胞。在BNST中，E2和DHT标记的神经元存在于所有亚区，而T标记仅限于BNST的前后内侧亚区。这些结果表明，在控制交配行为中传递化学感觉信号的核团中，含雄激素和雌激素受体的神经元分布有相当大的重叠。
