[雌二醇和孕酮对人颗粒细胞体外产生巨噬细胞集落刺激因子的影响]

[Effect of estradiol and progesterone on production of macrophage colony-stimulating factor by human granulosa cells in vitro].

作者信息

Zhang Zhi-fen, Xie Xiu-qin, Salmassi A, Mettler L

机构信息

Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hanzhou 310006, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2003 Sep;38(9):549-51.

PMID:14680610

Abstract

OBJECTIVE

To investigate the effect of estradiol (E(2)) and progesterone (P) on macrophage colony-stimulating factor (M-CSF) production by human luteinized granulosa cells in vitro.

METHODS

Human luteinized granulosa cells were isolated from follicular fluid of superovulated infertile patients undergoing intracytoplasmatic sperm injection (ICSI). Luteinized granulosa cells were cultured with HAM's F-10 medium with various concentrations of E(2) or P (0, 1 x 10(-7), 1 x 10(-6), 1 x 10(-5), 1 x 10(-4), 1 x 10(-3) mol/L). Media were removed at 72 h after culture. M-CSF in media was measured by a solid phase enzyme-linked immunosorbant assay (ELISA) and estradiol and progesterone in media were measured by enzyme immunoassays (EIA).

RESULTS

The basic concentration of M-CSF in cultivated granulosa cells without E(2) stimulation was low (47 +/- 15 ng/L). However, E(2) at concentrations of 1 x 10(-6) - 1 x 10(-3) mol/L caused a significant increase of M-CSF secretion by luteinized granulosa cells in a dose-dependent mode (2.3, 4.5, 6.9, 7.9 times higher than the basic level, respectively) (P < 0.05). E(2) at a concentration of 1 x 10(-7) mol/L, however, did not stimulate the production of M-CSF (P > 0.05). P at concentrations of 1 x 10(-7) - 1 x 10(-3) mol/L showed no effect on M-CSF production by luteinized granulosa cells (P > 0.05).

CONCLUSIONS

Estradiol can stimulate M-CSF production by luteinized granulosa cells in vitro in a dose-dependent mode, on the other hand P cannot induce M-CSF production by luteinized granulosa cells. The mechanism of estradiol regulating follicular development may partially via M-CSF/receptor pathway.

摘要

目的

研究雌二醇（E₂）和孕酮（P）对体外培养的人黄素化颗粒细胞产生巨噬细胞集落刺激因子（M-CSF）的影响。

方法

从接受卵胞浆内单精子注射（ICSI）的超排卵不孕患者的卵泡液中分离出人黄素化颗粒细胞。将黄素化颗粒细胞与含有不同浓度E₂或P（0、1×10⁻⁷、1×10⁻⁶、1×10⁻⁵、1×10⁻⁴、1×10⁻³mol/L）的HAM's F-10培养基一起培养。培养72小时后去除培养基。通过固相酶联免疫吸附测定（ELISA）测量培养基中的M-CSF，通过酶免疫测定（EIA）测量培养基中的雌二醇和孕酮。

结果

未受E₂刺激的培养颗粒细胞中M-CSF的基础浓度较低（47±15 ng/L）。然而，浓度为1×10⁻⁶ - 1×10⁻³mol/L的E₂以剂量依赖方式导致黄素化颗粒细胞分泌的M-CSF显著增加（分别比基础水平高2.3、4.5、6.9、7.9倍）（P<0.05）。然而，浓度为1×10⁻⁷mol/L的E₂并未刺激M-CSF的产生（P>0.05）。浓度为1×10⁻⁷ - 1×10⁻³mol/L的P对黄素化颗粒细胞产生M-CSF没有影响（P>0.05）。