Zhang Zhifen, Fang Qin, Wang Jianmin
Department of Gynecology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Fertil Steril. 2008 Sep;90(3):749-54. doi: 10.1016/j.fertnstert.2007.06.098. Epub 2007 Sep 19.
To evaluate the role of macrophage colony-stimulating factor (M-CSF) in steroidogenesis by luteinized granulosa cells (GCs) and to examine the effect of FSH, E(2), and P on the production of M-CSF by GCs.
In vitro experimental study.
University hospital with an IVF-intracytoplasmic sperm injection program.
PATIENT(S): Forty infertile women who underwent intracytoplasmatic sperm injection.
INTERVENTION(S): The GCs obtained from these patients during oocyte retrieval were cultured and then treated with different concentrations of M-CSF, FSH, E(2), or P for 72 hours.
MAIN OUTCOME MEASURE(S): The M-CSF concentrations were determined by ELISA, and the concentrations of E(2) and P were measured by enzyme immunoassay.
RESULT(S): The M-CSF secretion of GCs was enhanced by FSH or E(2) in vitro in a dose-dependent manner but was unaffected by P. On the other hand, M-CSF elicited a production of E(2) and P by GCs in a dose-dependent manner in the presence or absence of FSH.
CONCLUSION(S): The results suggest that the growth factor M-CSF might have an important role in ovarian function.
评估巨噬细胞集落刺激因子（M-CSF）在黄素化颗粒细胞（GCs）类固醇生成中的作用，并研究促卵泡激素（FSH）、雌二醇（E₂）和孕酮（P）对GCs分泌M-CSF的影响。
体外实验研究。
一家开展体外受精-卵胞浆内单精子注射项目的大学医院。
40例行卵胞浆内单精子注射的不孕女性。
在取卵时从这些患者获取GCs进行培养，然后用不同浓度的M-CSF、FSH、E₂或P处理72小时。
采用酶联免疫吸附测定法（ELISA）测定M-CSF浓度，采用酶免疫测定法测量E₂和P的浓度。
体外实验中，FSH或E₂可呈剂量依赖性增强GCs的M-CSF分泌，但P对其无影响。另一方面，无论有无FSH存在，M-CSF均可呈剂量依赖性诱导GCs产生E₂和P。
结果表明生长因子M-CSF可能在卵巢功能中发挥重要作用。
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