Tkachenko H M, Kurhaliuk N M, Vovkanych L S
Ivan Franko Lviv National University, Ukraine.
Ukr Biokhim Zh (1999). 2003 Sep-Oct;75(5):69-76.
Effects of ATP-sensitive potassium (KATP) channels opener pinacidil (0.06 mg/kg) and inhibitor glibenclamide (1 mg/kg) in rats with different resistance to hypoxia on indices of ADP-stimulation of mitochondrial respiration by Chance, calcium capacity and processes of lipid peroxidation in liver has been investigated. We used next substrates of oxidation: 0.35 mM succinate, 1 mM alpha-ketoglutarate. Additional analyses contain the next inhibitors: mitochondrial fermentative complex I-10 mkM rotenone, succinate dehydrogenase 2 mM malonic acid. It was shown that effects of pinacidil induced the increasing of oxidative phosporylation efficacy and ATP synthesis together with lowering of calcium capacity in rats with low resistance to hypoxia. Effects of pinacidil were leveled by glibenclamide. These changes are connected with the increasing of respiratory rate, calcium overload and intensification of lipid peroxidation processes. A conclusion was made about protective effect of pinacidil on mitochondrial functioning by economization of oxygen-dependent processes, adaptive potentialities of organisms with low resistance to hypoxia being increased.
研究了ATP敏感性钾（KATP）通道开放剂吡那地尔（0.06mg/kg）和抑制剂格列本脲（1mg/kg）对不同低氧耐受性大鼠的ADP刺激线粒体呼吸指标、钙容量及肝脏脂质过氧化过程的影响。我们使用了以下氧化底物：0.35mM琥珀酸、1mMα-酮戊二酸。另外的分析中还使用了以下抑制剂：线粒体呼吸链复合物I的抑制剂10μM鱼藤酮、琥珀酸脱氢酶的抑制剂2mM丙二酸。结果表明，吡那地尔可提高低氧耐受性低的大鼠的氧化磷酸化效率和ATP合成，同时降低其钙容量。格列本脲可消除吡那地尔的这些作用。这些变化与呼吸速率增加、钙超载及脂质过氧化过程增强有关。得出结论：吡那地尔通过节省氧依赖过程对线粒体功能具有保护作用，可增强低氧耐受性低的生物体的适应潜力。
