Tam N F Y, Guo C L, Yau C, Ke L, Wong Y S
Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
Water Sci Technol. 2003;48(8):177-83.
Surface sediments from two mangrove swamps in Hong Kong were enriched to obtain PAH-degrading microbial consortia. The consortium from sediments enriched with phenanthrene (Phe) in Ma Wan, a mangrove swamp next to a boat anchorage, was effective in degrading PAH with 96.7% and 89.9% Phe degradation at 0 and 10 ppt salinities, respectively. The Phe-enriched microbial consortium could also use pyrene (Pyr) as the sole carbon source but the degradation was much less (around 15%). On the other hand, the Phe-enriched consortium from sediments in Yi O, a mangrove swamp recently polluted by an oil spill, completely removed Phe and Pyr at both 0 and 10 ppt salinities. This suggests that different consortia were selected in two sediments using the same PAH substrate. Not only sediments, PAH substrate used for enrichment also selected different consortia. The Pyr- and Fla-enriched Yi O consortia only achieved 10.5 and 4.5% Phe degradation, respectively and the corresponding pyrene degradation was 7 and 40%. Among the three PAH compounds, fluoranthene (Fla) was most difficult to degrade, and 21.1, 11.3, and 36.8% Fla were degraded by Phe-, Fla- and Pyr-enriched cultures, respectively.
对香港两个红树林湿地的表层沉积物进行富集，以获得多环芳烃降解微生物群落。在靠近船只停泊处的马湾红树林湿地，用菲（Phe）富集沉积物得到的微生物群落，在盐度为0和10ppt时对多环芳烃具有高效降解能力，菲的降解率分别为96.7%和89.9%。以菲富集的微生物群落也能利用芘（Pyr）作为唯一碳源，但降解率要低得多（约15%）。另一方面，在最近受溢油污染的荔枝澳红树林湿地沉积物中，以菲富集的微生物群落在盐度为0和10ppt时都能完全去除菲和芘。这表明，使用相同的多环芳烃底物，在两种沉积物中选择出了不同的微生物群落。不仅沉积物会影响选择结果，用于富集的多环芳烃底物也会选择出不同的微生物群落。在荔枝澳，以芘和荧蒽（Fla）富集得到的微生物群落对菲的降解率分别仅为10.5%和4.5%，相应的芘降解率分别为7%和40%。在这三种多环芳烃化合物中，荧蒽最难降解，以菲、荧蒽和芘富集培养的微生物群落对荧蒽的降解率分别为21.1%、11.3%和36.8%。
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