Cen D, Levin J
Department of Laboratory Medicine, University of California School of Medicine, San Francisco.
Exp Hematol. 1992 Oct;20(9):1094-100.
The cell cycle status of megakaryocyte colony-forming cells (Meg-CFC) and granulocyte-macrophage colony-forming cells (GM-CFC) from the spleen and bone marrow of C57BL mice was evaluated by determining the effects of hydroxyurea (OHU) or cytosine arabinoside (Ara-C), both in vivo and in vitro, upon colony-forming cells (CFC). The concentrations of cells in culture (2 x 10(6) to 4 x 10(6)/ml for spleen and 0.25 x 10(5) to 1.0 x 10(5)/ml for bone marrow) did not alter cell cycle status of either Meg-CFC or GM-CFC. Determination of cell cycle status following in vivo administration of OHU indicated that 25.2% of Meg-CFC and 28.1% of GM-CFC in the spleen, and 26.0% of Meg-CFC and 29.5% of GM-CFC in the bone marrow, were in cycle. In vitro incubation of CFC with OHU showed that in the spleen 25.1% of Meg-CFC and 24.2% of GM-CFC were engaged in DNA synthesis, whereas in bone marrow 28.5% of Meg-CFC and 29.2% of GM-CFC were synthesizing DNA. Incubation with Ara-C, in vitro, gave similar results, with 26.0% of Meg-CFC and 26.2% of GM-CFC in the spleen, and 27.1% of Meg-CFC and 31.4% of GM-CFC in the bone marrow, in cycle. In summary, significant differences were not observed between the cell cycle status of Meg-CFC and GM-CFC, whether derived from spleen or bone marrow. In vitro and in vivo measurements (with OHU) and in vitro measurements with two cytotoxic drugs (OHU versus Ara-C) also provided similar results. The data suggest that the regulation of DNA synthesis in both Meg-CFC and GM-CFC in the murine spleen and bone marrow is similar.
通过测定羟基脲（OHU）或阿糖胞苷（Ara-C）在体内和体外对集落形成细胞（CFC）的影响，评估了C57BL小鼠脾脏和骨髓中巨核细胞集落形成细胞（Meg-CFC）和粒细胞-巨噬细胞集落形成细胞（GM-CFC）的细胞周期状态。培养物中的细胞浓度（脾脏为2×10⁶至4×10⁶/ml，骨髓为0.25×10⁵至1.0×10⁵/ml）并未改变Meg-CFC或GM-CFC的细胞周期状态。体内给予OHU后细胞周期状态的测定表明，脾脏中25.2%的Meg-CFC和28.1%的GM-CFC，以及骨髓中26.0%的Meg-CFC和29.5%的GM-CFC处于细胞周期中。CFC与OHU在体外孵育显示，脾脏中25.1%的Meg-CFC和24.2%的GM-CFC参与DNA合成，而骨髓中28.5%的Meg-CFC和29.2%的GM-CFC正在合成DNA。体外与Ara-C孵育得到类似结果，脾脏中26.0%的Meg-CFC和26.2%的GM-CFC，以及骨髓中27.1%的Meg-CFC和31.4%的GM-CFC处于细胞周期中。总之，无论是来自脾脏还是骨髓，Meg-CFC和GM-CFC的细胞周期状态之间未观察到显著差异。体外和体内测量（使用OHU）以及使用两种细胞毒性药物（OHU与Ara-C）的体外测量也提供了类似结果。数据表明，小鼠脾脏和骨髓中Meg-CFC和GM-CFC的DNA合成调节相似。
