用大鼠胆碱乙酰转移酶cDNA转染的神经母细胞瘤x胶质瘤杂交NG108-15细胞中乙酰胆碱分泌增强。

Enhanced acetylcholine secretion in neuroblastoma x glioma hybrid NG108-15 cells transfected with rat choline acetyltransferase cDNA.

作者信息

Kimura Y, Oda Y, Deguchi T, Higashida H

机构信息

Department of Biophysics, Kanazawa University School of Medicine, Japan.

出版信息

FEBS Lett. 1992 Dec 21;314(3):409-12. doi: 10.1016/0014-5793(92)81516-o.

DOI:10.1016/0014-5793(92)81516-o

PMID:1468577

Abstract

Neuroblastoma x glioma hybrid NG108-15 cells and mouse neuroblastoma N18TG-2 and N1E-115 cells were transiently transfected with the sense cDNA coding for rat choline acetyltransferase (ChAT). All transfected cell lines showed a high level of ChAT activity. ACh secretion was monitored by recording miniature end-plate potentials (MEPPs) in striated muscle cells that had been co-cultured with transfected cells. The number of muscle cells with synaptic responses and the MEPP frequency were higher in co-culture with transfected NG108-15 cells than with control or mock cells. No synaptic response was detected in muscle cells co-cultured with transfected N18TG-2 or N1E-115 cells. The results show that ACh secretion into the synaptic cleft was enhanced due to ChAT overexpression in NG108-15 hybrid cells but not in neuroblastoma cells.

摘要

将编码大鼠胆碱乙酰转移酶（ChAT）的正义cDNA瞬时转染至神经母细胞瘤x胶质瘤杂交NG108 - 15细胞以及小鼠神经母细胞瘤N18TG - 2和N1E - 115细胞。所有转染的细胞系均表现出高水平的ChAT活性。通过记录与转染细胞共培养的横纹肌细胞中的微小终板电位（MEPPs）来监测乙酰胆碱（ACh）的分泌。与转染的NG108 - 15细胞共培养的肌肉细胞中，出现突触反应的肌肉细胞数量和MEPP频率高于与对照或模拟转染细胞共培养的情况。在与转染的N18TG - 2或N1E - 115细胞共培养的肌肉细胞中未检测到突触反应。结果表明，由于NG108 - 15杂交细胞中ChAT过表达，突触间隙中的ACh分泌增加，而神经母细胞瘤细胞中则未出现这种情况。