Kato Shingo, Saito Rie, Hiraishi Yoshiyuki, Kitamura Naoto, Matsumoto Tomoko, Hanabusa Hideji, Kamakura Mitsuhiro, Ikeda Yasuo, Negishi Masayoshi
Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan.
AIDS Res Hum Retroviruses. 2003 Nov;19(11):1057-63. doi: 10.1089/088922203322588431.
We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain re-action (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97% of samples. Sixty-five subjects were infected with subtype B, 16 with CRFO1_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype Band 12 with CRFO1_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRFO1_AE are evenly predominant in a heterosexually transmitted group.
我们对日本东京都地区三家参考医院的89名HIV-1感染者进行了HIV-1亚型鉴定。采用区分A、B、C和CRF01_AE亚型的特异性聚合酶链反应（PCR）及HIV-1 env C2V3C3序列的系统发育分析进行亚型鉴定。特异性PCR在97%的样本中得到明确结果。65名受试者感染B亚型，16名感染CRF01_AE，4名感染A亚型，1名感染CRF02_AG，3名感染C亚型。在31名通过异性接触感染的日本个体中，13名感染B亚型，12名感染CRF01_AE。41名通过同性接触感染的日本男性均感染B亚型。这些结果表明，B亚型在同性传播群体中占绝对优势，而在异性传播群体中B亚型和CRF01_AE亚型占比相当。
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