Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital, China Medical University, Shenyang, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
J Med Virol. 2015 Apr;87(4):557-68. doi: 10.1002/jmv.24116.
To investigate the molecular subtypes of prevalent HIV-1 strains and characterize the genetics of dominant strains among men who have sex with men. Molecular epidemiology surveys in this study concentrated on the prevalent HIV-1 strains in Liaoning province by year. 229 adult patients infected with HIV-1 and part of a high-risk group of men who have sex with men were recruited. Reverse transcription and nested PCR amplification were performed. Sequencing reactions were conducted and edited, followed by codon-based alignment. NJ phylogenetic tree analyses detected two distinct CRF01_AE phylogenetic clusters, designated clusters 1 and 2. Clusters 1 and 2 accounted for 12.8% and 84.2% of sequences in the pol gene and 17.6% and 73.1% of sequences in the env gene, respectively. Another six samples were distributed on other phylogenetic clusters. Cluster 1 increased significantly from 5.6% to 20.0%, but cluster 2 decreased from 87.5% to 80.0%. Genetic distance analysis indicated that CRF01_AE cluster 1 in Liaoning was homologous to epidemic CRF01_AE strains, but CRF01_AE cluster 2 was different from other scattered strains. Additionally, significant differences were found in tetra-peptide motifs at the tip of V3 loop between cluster 1 and 2; however, differences in coreceptor usage were not detected. This study shows that subtype CRF01_AE strain may be the most prevalent epidemic strain in the men who have sex with men. Genetic characteristics of the subtype CRF01_AE cluster strain in Liaoning showed homology to the prevalent strains of men who have sex with men in other parts of China.
为了研究流行的 HIV-1 毒株的分子亚型，并分析男男性行为人群中优势毒株的遗传特征。本研究的分子流行病学调查主要集中在辽宁省流行的 HIV-1 毒株。共招募了 229 名感染 HIV-1 的成年患者和部分男男性行为者高危人群。进行逆转录和巢式 PCR 扩增。进行测序反应并编辑，然后进行基于密码子的对齐。NJ 系统发育树分析检测到两个不同的 CRF01_AE 系统发育簇，分别命名为簇 1 和簇 2。簇 1 和簇 2 分别占 pol 基因序列的 12.8%和 84.2%，env 基因序列的 17.6%和 73.1%。另外 6 个样本分布在其他系统发育簇中。簇 1 从 5.6%显著增加到 20.0%，但簇 2 从 87.5%下降到 80.0%。遗传距离分析表明，辽宁的 CRF01_AE 簇 1 与流行的 CRF01_AE 株同源，但 CRF01_AE 簇 2 与其他散在株不同。此外，在 V3 环尖端的四肽基序中，簇 1 和簇 2 之间存在显著差异；然而，未检测到核心受体使用的差异。本研究表明，CRF01_AE 亚型株可能是男男性行为人群中最流行的流行株。辽宁 CRF01_AE 亚系株的遗传特征与中国其他地区男男性行为者的流行株具有同源性。
