Yan Ji-Jing, Kim Do-Hoon, Moon Yoo-Sun, Jung Jun-Sub, Ahn Eun-Mi, Baek Nam-In, Song Dong-Keun
Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University, Chunchon, Kangwon-Do, 200-702, South Korea.
Prog Neuropsychopharmacol Biol Psychiatry. 2004 Jan;28(1):25-30. doi: 10.1016/S0278-5846(03)00168-4.
We investigated the effect of long-term oral administration of ethanolic extract of Angelica gigas Nakai (Umbelliferae) (EAG) or decursinol, a coumarin isolated from A. gigas, on beta-amyloid peptide 1-42 (Abeta(1-42))-induced memory impairment in mice. Mice were allowed free access to drinking water (control) or water containing different concentrations of EAG. After 4 weeks, Abeta(1-42) (410 pmol) was administered via intracerebroventricular injection. Pretreatment of mice with EAG (0.1%) for 4 weeks significantly blocked the Abeta(1-42)-induced impairment in passive avoidance performance. Next, mice were fed with chow mixed with various doses of decursinol for 4 weeks before intracerebroventricular injection of Abeta(1-42) (410 pmol). Pretreatment of mice with decursinol (0.001%, 0.002%, and 0.004%) for 4 weeks significantly attenuated the Abeta(1-42)-induced impairment in passive avoidance performance. Decursinol (0.004%) also significantly blunted the Abeta(1-42)-induced decrease in alternation behavior (spatial working memory) in the Y-maze test without change in general locomotor activity. These findings suggest that EAG or decursinol may have preventive effect against memory impairment related with Abeta of Alzheimer's disease.
我们研究了长期口服当归(伞形科)乙醇提取物(EAG)或从当归中分离出的香豆素蛇床子素对β-淀粉样肽1-42(Aβ(1-42))诱导的小鼠记忆损伤的影响。让小鼠自由饮用饮用水(对照组)或含有不同浓度EAG的水。4周后,通过脑室内注射给予Aβ(1-42)(410 pmol)。用EAG(0.1%)对小鼠进行4周预处理可显著阻断Aβ(1-42)诱导的被动回避行为损伤。接下来,在脑室内注射Aβ(1-42)(410 pmol)前,给小鼠喂食含不同剂量蛇床子素的食物4周。用蛇床子素(0.001%、0.002%和0.004%)对小鼠进行4周预处理可显著减轻Aβ(1-42)诱导的被动回避行为损伤。蛇床子素(0.004%)在Y迷宫试验中也显著减弱了Aβ(1-42)诱导的交替行为(空间工作记忆)下降,而不改变总体运动活性。这些发现表明,EAG或蛇床子素可能对与阿尔茨海默病Aβ相关的记忆损伤具有预防作用。