Han Na-Ra, Park Hi-Joon, Ko Seong-Gyu, Moon Phil-Dong
College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Foods. 2023 Jan 6;12(2):276. doi: 10.3390/foods12020276.
An herbal mixture (SH003) of , , and exhibits therapeutic effects on carcinomas and immunosuppression. However, the role of JRP-SNF102, which is an advanced mixture of SH003, in regulating inflammatory responses is unexplored. We aim to substantiate the therapeutic potential of JRP-SNF102 and its active component, formononetin (FMN), as a functional food that moderates inflammatory responses. The inhibitory effects of JRP-SNF102 or FMN on thymic stromal lymphopoietin (TSLP) levels were evaluated in phorbol 12-myristate 13-acetate (PMA) plus A23187-activated human mast cell line-1 (HMC-1) cells and a mouse model of PMA-induced ear edema. The JRP-SNF102 or FMN inhibited the secretion and mRNA expression of TSLP and vascular endothelial growth factor (VEGF) in the activated HMC-1 cells. The expression levels of murine double minute 2 (MDM2), hypoxia-inducible factor 1α (HIF1α), and NF-κB were also suppressed by JRP-SNF102 or FMN in the activated HMC-1 cells. The JRP-SNF102 or FMN inhibited TSLP and VEGF levels, attenuating redness and ear thickness in mice with acute ear edema; JRP-SNF102 or FMN reduced the expression levels of MDM2, HIF1α, and NF-κB in the ear tissues. These findings suggest the potential for JRP-SNF102 as a functional food in the treatment of inflammatory skin disorders through suppression of TSLP and VEGF.
由[具体成分1]、[具体成分2]和[具体成分3]组成的草药混合物（SH003）对癌症和免疫抑制具有治疗作用。然而，作为SH003的改良混合物，JRP - SNF102在调节炎症反应中的作用尚未得到研究。我们旨在证实JRP - SNF102及其活性成分芒柄花黄素（FMN）作为调节炎症反应的功能性食品的治疗潜力。在佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）加A23187激活的人肥大细胞系 - 1（HMC - 1）细胞和PMA诱导的小鼠耳部水肿模型中，评估了JRP - SNF102或FMN对胸腺基质淋巴细胞生成素（TSLP）水平的抑制作用。JRP - SNF102或FMN抑制了活化的HMC - 1细胞中TSLP和血管内皮生长因子（VEGF）的分泌及mRNA表达。在活化的HMC - 1细胞中，JRP - SNF102或FMN也抑制了小鼠双微体2（MDM2）、缺氧诱导因子1α（HIF1α）和核因子κB（NF - κB）的表达水平。JRP - SNF102或FMN抑制了TSLP和VEGF水平，减轻了急性耳部水肿小鼠的发红和耳部厚度；JRP - SNF102或FMN降低了耳部组织中MDM2、HIF1α和NF - κB的表达水平。这些发现表明，JRP - SNF102作为功能性食品，通过抑制TSLP和VEGF，在治疗炎症性皮肤病方面具有潜力。
