克罗地亚亚急性硬化性全脑炎病例中麻疹病毒株的检测与特征分析。
Detection and characterization of measles virus strains in cases of subacute sclerosing panencephalitis in Croatia.
作者信息
Forcić Dubravko, Baricević Marijana, Zgorelec Renata, Kruzić Vjekoslava, Kaić Bernard, Marina Branka Marusić Della, Sojat Ljerka Cvitanović, Tesović Goran, Mazuran Renata
机构信息
Department of Molecular Biomedicine, Institute of Immunology Inc., Rockefellerova 10, Zagreb 10000, Croatia.
出版信息
Virus Res. 2004 Jan;99(1):51-6. doi: 10.1016/j.virusres.2003.10.003.
Two cases of subacute sclerosing panencephalitis (SSPE), diagnosed in Croatia in 2002, were investigated. The coding regions of the matrix (M), hemagglutinin (H) and nucleoprotein (N) genes of measles virus were sequenced following direct RT-PCR amplification of viral RNA extracted from brain tissue. Phylogenetic analysis of the sequences of H and N genes, showed that both strains belonged to genotype D6. No vaccine strain was detected although both patients had been previously immunized. The comparison of analyzed sequences of two SSPE causative viruses with corresponding sequences of D6 genotype and with each other revealed a number of mutations in N and H gene sequences. In comparison to the Edmonston reference strain, the M gene of the SSPE viruses showed the characteristic biased hypermutation and a premature termination codon in one of the patients.
对2002年在克罗地亚诊断出的两例亚急性硬化性全脑炎（SSPE）病例进行了调查。从脑组织中提取病毒RNA，经直接逆转录聚合酶链反应（RT-PCR）扩增后，对麻疹病毒的基质（M）、血凝素（H）和核蛋白（N）基因的编码区进行测序。H和N基因序列的系统发育分析表明，两株病毒均属于D6基因型。尽管两名患者此前均已接种疫苗，但未检测到疫苗株。将两株SSPE致病病毒的分析序列与D6基因型的相应序列以及彼此进行比较，发现N和H基因序列存在一些突变。与埃德蒙斯顿参考株相比，其中一名患者的SSPE病毒M基因表现出特征性的偏向性超突变和一个提前终止密码子。
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