Kühne Simmonds M, Brown D W G, Jin L
Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK.
Virol J. 2006 Jun 21;3:49. doi: 10.1186/1743-422X-3-49.
Subacute sclerosing panencephalitis (SSPE) is a rare, slowly progressive neurological disorder caused by the persistent infection with measles virus (MV). Despite much research into SSPE, its pathology remains obscure. We examined autopsy tissues of eight SSPE patients by real time quantitative PCR, immunohistochemistry and immunoblotting to determine viral load. MV N, M and H gene RNA could be detected in the central nervous system (CNS) of all patients and in two non-CNS tissues of one patient. The viral burden between patients differed up to four-fold by quantitative PCR and corresponded with detection of MV protein. The level of both viral RNA and antigen in the brain may correlate with disease progression.
亚急性硬化性全脑炎（SSPE）是一种由麻疹病毒（MV）持续感染引起的罕见、进展缓慢的神经疾病。尽管对SSPE进行了大量研究，但其病理学仍不清楚。我们通过实时定量PCR、免疫组织化学和免疫印迹法检查了8例SSPE患者的尸检组织，以确定病毒载量。在所有患者的中枢神经系统（CNS）以及1例患者的两个非CNS组织中均可检测到MV N、M和H基因RNA。通过定量PCR检测，患者之间的病毒载量相差高达4倍，且与MV蛋白的检测结果相符。脑中病毒RNA和抗原的水平可能与疾病进展相关。
