Vosková D, Váleková L, Fedorová J, Hudecek J, Kubisz P
Clinic of Hematology and Transfusiology, Faculty Hospital of Martin and Jessenius Medical Faculty, Comenius University, Martin, Slovak Republic.
Neoplasma. 2003;50(6):422-7.
The aim of this study was to evaluate the heterogeneity of immunophenotype features in acute leukemia patients and to detect the presence of leukemia-associated immunophenotypes. We prospectively investigated the phenotype of blast cells from 44 adult acute leukemia patients using a large panel of monoclonal antibodies by multiparametric flow cytometry. Thirty-three patients were classified as AML according to the FAB classification. Eleven patients were diagnosed as ALL (10 cases B-ALL, 1 case T-ALL) according to both FAB and immunnophenotyping. We found leukemia-associated phenotypes in 28 of 33 AML patients (84.8%) and in 8 of 11 ALL patients (72.7%). In 61.1% of patients more than one aberrant phenotype was observed. Linear infidelity was the most frequent aberrancy in both AML (64.3%) and ALL (37.5%) subgroups. The present study shows that MFC is a helpful method for sufficient identification of leukemic cells and for determination of blast cells immunophenotype heterogeneity. The double stain flow cytometry in our study revealed aberrant phenotypes in up to 81.8% patients.
本研究的目的是评估急性白血病患者免疫表型特征的异质性，并检测白血病相关免疫表型的存在。我们通过多参数流式细胞术，使用大量单克隆抗体对44例成年急性白血病患者的原始细胞表型进行了前瞻性研究。根据FAB分类，33例患者被归类为AML。根据FAB分类和免疫表型分析，11例患者被诊断为ALL（10例B-ALL，1例T-ALL）。我们在33例AML患者中的28例（84.8%）和11例ALL患者中的8例（72.7%）发现了白血病相关表型。在61.1%的患者中观察到一种以上异常表型。线性不忠实是AML（64.3%）和ALL（37.5%）亚组中最常见的异常。本研究表明，MFC是充分识别白血病细胞和确定原始细胞免疫表型异质性的有用方法。我们研究中的双染流式细胞术在高达81.8%的患者中发现了异常表型。
