使用等位基因特异性引物聚合酶链反应（PCR-ASP）对法国巴斯克人进行达菲血型基因分型。

Duffy blood group genotyping in French Basques using polymerase chain reaction with allele-specific primers (PCR-ASP).

作者信息

Bauduer Frédéric, Touinssi Mhammed, Degioanni Anna, Leroux Stéphane, Dutour Olivier, Ducout Louis, De Micco Philippe, Chiaroni Jacques

机构信息

UMR CNRS 6578, Université de la Méditerranée, Unité d'Anthropologie - Adaptabilité biologique et culturelle, Faculté de Médecine de Marseille, 13385 Marseille, France.

出版信息

Am J Hum Biol. 2004 Jan-Feb;16(1):78-81. doi: 10.1002/ajhb.10230.

DOI:10.1002/ajhb.10230

PMID:14689518

Abstract

Blood group antigens such as Duffy represent interesting models for population genetics studies. The distribution of the Duffy blood group was determined using PCR in a sample of Basque (n = 126) and non-Basque (n = 110) patients from the general hospital of the French Basque Country. The frequency of FYA allele was significantly lower among autochthonous French Basques (P < 0.001). This result, obtained for the first time by PCR analysis in this population, was within the range of previous historical studies in various Basque subpopulations using traditional hemagglutination methods. When compared with European data, our Basque sample demonstrated the lowest FYA/FYB ratio, this fact confirming hemotypology data published before the molecular biology era. Our results with FYX were quite similar to those reported in European populations.

摘要

诸如达菲血型抗原等血型抗原是群体遗传学研究的有趣模型。在法国巴斯克地区综合医院的一组巴斯克患者（n = 126）和非巴斯克患者（n = 110）样本中，使用聚合酶链反应（PCR）确定了达菲血型的分布。FYA等位基因的频率在法国本土巴斯克人中显著较低（P < 0.001）。这一首次通过该群体的PCR分析获得的结果，在以往使用传统血凝方法对不同巴斯克亚群体进行的历史研究范围内。与欧洲数据相比，我们的巴斯克样本显示出最低的FYA/FYB比率，这一事实证实了分子生物学时代之前发表的血型学数据。我们关于FYX的结果与欧洲人群中报道的结果非常相似。