Kinikli G, Ateş A, Turgay M, Akay G, Kinikli S, Tokgöz G
Department of Clinical Immunology and Rheumatology, Ankara University Medical School, Ankara, Turkey.
Scand J Rheumatol. 2003;32(5):277-80. doi: 10.1080/03009740310003901.
Association with human leukocyte antigen (HLA)-DRB alleles, implicated in the aetiopathogenesis of rheumatoid arthritis (RA), is found to be different in various ethnic groups. This study aimed to investigate DRB1 alleles in RA patients in Turkey, and to examine the effect of these alleles on disease severity.
We performed PCR-based DRBI genotyping of 104 RA patients recruited from clinical settings and 110 healthy controls. HLA DRB1 alleles frequencies in RA patients and healthy controls were determined. Phenotype frequencies of patients and controls were compared. Disease severity was assessed by radiological erosion, presence of extra-articular involvement, and functional index.
Significant differences were in the frequencies of DRB104 (46.2% versus 20.9%, p < 0.001), DRB10401 (10.6% versus 0%, p < 0.001), DRB10405 (8.7% versus 0%, p = 0.001), DRB1 0404 (15.4% versus 3.6%, p < 0.01), DRB101 (21.2% versus 10.9%, p < 0.05) and DRB10101 (16.3% versus 5.5%, p = 0.01) between RA patients and controls. HLA-DRB1 alleles did not show any association with seropositivity, extra-articular involvement, radiological erosion, or functional index.
Our results suggest that the HLA-DRB1 alleles, particularly HLA-DRB1*04 and subtypes, were associated with RA.
与类风湿关节炎（RA）发病机制相关的人类白细胞抗原（HLA）-DRB等位基因在不同种族中存在差异。本研究旨在调查土耳其RA患者的DRB1等位基因，并研究这些等位基因对疾病严重程度的影响。
我们对从临床招募的104例RA患者和110例健康对照进行了基于PCR的DRB1基因分型。确定RA患者和健康对照中HLA DRB1等位基因频率。比较患者和对照的表型频率。通过放射学侵蚀、关节外受累情况和功能指数评估疾病严重程度。
RA患者与对照之间，DRB104（46.2%对20.9%，p<0.001）、DRB10401（10.6%对0%，p<0.001）、DRB10405（8.7%对0%，p = 0.001）、DRB10404（15.4%对3.6%，p<0.01）、DRB101（21.2%对10.9%，p<0.05）和DRB10101（16.3%对5.5%，p = 0.01）的频率存在显著差异。HLA-DRB1等位基因与血清阳性、关节外受累、放射学侵蚀或功能指数无任何关联。
我们的结果表明，HLA-DRB1等位基因，尤其是HLA-DRB1*04及其亚型，与RA相关。
