Brown C C, Olander H J, Senne D A
Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, USDA, Greenport, NY 11944.
J Comp Pathol. 1992 Oct;107(3):341-8. doi: 10.1016/0021-9975(92)90009-j.
Eighteen specific pathogen-free chickens (nine hens older than 1 year and nine 15-week-old males) were inoculated with highly pathogenic avian influenza virus A/Chicken/Pennsylvania/1370/1983 (H5N2). Birds were serially killed and tissues collected for histological and immunohistochemical evaluation. In the group of older hens, disease was acute or peracute. By immunohistochemistry, antigen was abundant in capillary endothelium in multiple organs, and staining for antigen in parenchymal cells was marked in brain and heart. In the group of younger male birds, disease was subacute. Immunohistochemical staining of capillary endothelium was less pronounced and viral antigen staining was evident in the parenchymal cells of the heart, brain and kidney.
18只特定病原体-free鸡（9只1岁以上母鸡和9只15周龄雄性鸡）接种高致病性甲型禽流感病毒A/鸡/宾夕法尼亚/1370/1983（H5N2）。依次对鸡进行宰杀，并收集组织用于组织学和免疫组织化学评估。在年龄较大的母鸡组中，疾病为急性或超急性。通过免疫组织化学，多个器官的毛细血管内皮中抗原丰富，实质细胞中的抗原染色在脑和心脏中明显。在较年轻的雄性鸡组中，疾病为亚急性。毛细血管内皮的免疫组织化学染色不太明显，病毒抗原染色在心脏、脑和肾的实质细胞中明显。
