Lombardi Rossana, Chantarangkul Veena, Cattaneo Marco, Tripodi Armando
Department of Internal Medicine, Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, University and IRCCS Maggiore Hospital, Via Pace 9, Milan 20122, Italy.
Thromb Res. 2003;111(4-5):281-4. doi: 10.1016/j.thromres.2003.09.006.
The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p<0.0001) or 0.25 (p=0.079).
需要测定血浆华法林水平，以调查患者是否不遵医嘱、是否存在抗凝抵抗、药物代谢及药代动力学情况。目前所描述的方法是基于从血浆中提取华法林，然后进行反相高效液相色谱分析。提取是关键步骤，可采用液相或固相提取。后者需要制备柱子，这使得操作过程存在变数。我们研究了即用型商业柱用于样品制备的适用性。该方法批间变异系数为11.8%。回收率为99%。50例患者的华法林浓度与每周剂量或国际标准化比值（INR）之间的相关系数分别为0.55（p<0.0001）或0.25（p=0.079）。
