Bjornson R M, Glocker B, Rohrmann G F
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-6502.
J Gen Virol. 1992 Dec;73 ( Pt 12):3177-83. doi: 10.1099/0022-1317-73-12-3177.
The DNA polymerase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was cloned and sequenced. The predicted DNA polymerase protein (1113 amino acids, 115.9K) was found to have an amino acid identity of 48% with the corresponding gene of the Autographa californica MNPV (AcMNPV). It contains five domains associated with substrate binding, primase interaction, and pyrophosphate hydrolysis and three domains associated with 3'-->5' exonuclease activity common to other DNA polymerases. A region with a conserved TATA promoter and a CAGT mRNA start site sequence motif was identified and shown to be transcribed by RNA polymerase II, indicating that the LdMNPV DNA polymerase gene is expressed as an early gene. An open reading frame possibly expressed as a late gene, oriented in the opposite direction and overlapping the N-terminal coding region of the DNA polymerase gene was found in the LdMNPV sequence and was shown to be conserved in the same position in AcMNPV.
克隆并测序了舞毒蛾多粒包埋核型多角体病毒（LdMNPV）的DNA聚合酶基因。预测的DNA聚合酶蛋白（1113个氨基酸，115.9K）与苜蓿银纹夜蛾核型多角体病毒（AcMNPV）的相应基因的氨基酸同一性为48%。它含有与底物结合、引发酶相互作用和焦磷酸水解相关的五个结构域，以及与其他DNA聚合酶共有的3'→5'核酸外切酶活性相关的三个结构域。鉴定出一个具有保守TATA启动子和CAGT mRNA起始位点序列基序的区域，并表明其由RNA聚合酶II转录，这表明LdMNPV DNA聚合酶基因作为早期基因表达。在LdMNPV序列中发现了一个可能作为晚期基因表达的开放阅读框，其方向相反且与DNA聚合酶基因的N端编码区重叠，并表明在AcMNPV的相同位置保守。
