Zhang Meibian, Lu Deqiang, He Jiliang, Jin Lifen
Institute of Occupational Health and Environmental Health, School of Medicine, Zhejiang University, Hangzhou 310031, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2002 Aug;20(4):273-6.
To study that low-intensity microwave whether or not enhances the genotoxic effects of mitomycin C(MMC) on human lymphocytes.
Single strand DNA breaks and chromosomal aberrations were measured by comet assay and cytokinesis-blocked micronucleus(CBMN) test in vitro when human lymphocytes were exposed to 2,450-MHz microwave (5.0 mW/cm2) alone and in combination with mitomycin C.
In the comet assay, the average comet lengths of microwave group[(29.1 +/- 8.1) micron in male and (25.9 +/- 7.5) micron in female] were not significantly different from those of control groups [(26.3 +/- 6.6) and (24.1 +/- 4.3) micron respectively] (P > 0.05). The average comet lengths of MMC group(0.0125, 0.0250, 0.0500, 0.1000 microgram/ml) were significantly longer than those of control groups (P < 0.01) and were increased with the dose of MMC. The average comet lengths of microwave combined with MMC (MW + MMC) also were increased with the doses of MMC and were significantly longer than those of control groups (P < 0.01). When MMC was > or = 0.0250 microgram/ml, microwave and MMC synergistically increased the single strand DNA breaks. In the micronucleus test, the average micronucleus rates of microwave groups were not higher than those of control groups (P > 0.05). The average micronucleus rates of MMC groups and MW + MMC groups were significantly higher than those of control groups (P < 0.01) when MMC was > or = 0.0500 microgram/ml. The average micronucleus rates of MW + MMC groups seemed higher than those of corresponding MMC groups, however the difference was not significant (P > 0.05).
Low-intensity(2,450-MHz) microwave did not induce DNA and chromosome damages on human lymphocytes, but enhanced the effects of DNA breaks induced by MMC.
研究低强度微波是否会增强丝裂霉素C（MMC）对人淋巴细胞的遗传毒性作用。
当人淋巴细胞单独暴露于2450 MHz微波（5.0 mW/cm²）以及与丝裂霉素C联合暴露时，通过彗星试验和胞质分裂阻滞微核（CBMN）试验在体外检测单链DNA断裂和染色体畸变情况。
在彗星试验中，微波组男性的平均彗星长度[（29.1±8.1）μm]和女性的平均彗星长度[（25.9±7.5）μm]与对照组男性[（26.3±6.6）μm]和女性[（24.1±4.3）μm]相比，差异无统计学意义（P>0.05）。MMC组（0.0125、0.0250、0.0500、0.1000 μg/ml）的平均彗星长度显著长于对照组（P<0.01），且随MMC剂量增加而增加。微波联合MMC（MW+MMC）组的平均彗星长度也随MMC剂量增加而增加，且显著长于对照组（P<0.01）。当MMC≥0.0250 μg/ml时，微波和MMC协同增加单链DNA断裂。在微核试验中，微波组的平均微核率不高于对照组（P>0.05）。当MMC≥0.0500 μg/ml时，MMC组和MW+MMC组的平均微核率显著高于对照组（P<0.01）。MW+MMC组的平均微核率似乎高于相应的MMC组，但差异无统计学意义（P>0.05）。
低强度（2450 MHz）微波未诱导人淋巴细胞的DNA和染色体损伤，但增强了MMC诱导的DNA断裂效应。
