Hansteen Inger-Lise, Lågeide Lars, Clausen Kjell Oskar, Haugan Vera, Svendsen Marit, Eriksen Jan Gunnar, Skiaker Rolf, Hauger Egil, Vistnes Arnt Inge, Kure Elin H
Department of Laboratory Medicine, Section of Medical Genetics, Telemark Hospital, Skien, Norway.
Anticancer Res. 2009 Aug;29(8):2885-92.
There are few cell studies on the direct genotoxic effects of microwave radiation. In this study, cytogenetic effects of microwave radiation alone or in combination with mitomycin C (MMC) were investigated.
Lymphocytes from two smoking and four non-smoking donors were exposed for 53 hours in vitro to 1.0 W/m(2) continuous-wave radiation at 18.0 GHz or 10 W/m(2) pulsed-wave at 16.5 GHz, alone or in combination with MMC. DNA synthesis and repair were inhibited in vitro in some cultures.
No synergistic effect was observed in cells exposed to combinations of microwave radiation and in vitro exposure to MMC, or to cells pre-exposed in vivo to tobacco smoke. For the 16.5 GHz pulsed exposure, a non-significant trend consisting of an increase in aberration frequencies with microwave radiation was shown for the DNA synthesis and repair inhibited cultures both with and without MMC.
Neither 18.0 GHz continuous-wave nor 16.5 GHz pulsed-wave exposure to human lymphocytes in vitro induced statistically significant increases in chromosomal aberration frequencies. 16.5 GHz pulsed-wave exposure requires further documentation before a true negative conclusion can be drawn.
关于微波辐射直接遗传毒性作用的细胞研究较少。在本研究中，对单独微波辐射或微波辐射与丝裂霉素C（MMC）联合作用的细胞遗传学效应进行了研究。
来自两名吸烟者和四名不吸烟者供体的淋巴细胞在体外分别暴露于18.0GHz的1.0W/m²连续波辐射或16.5GHz的10W/m²脉冲波辐射53小时，单独或与MMC联合暴露。在一些培养物中体外抑制DNA合成和修复。
在暴露于微波辐射与体外暴露于MMC的联合作用的细胞中，或在体内预先暴露于烟草烟雾的细胞中，未观察到协同效应。对于16.5GHz脉冲暴露，在有和没有MMC的DNA合成和修复抑制培养物中，均显示出微波辐射导致畸变频率增加的不显著趋势。
体外18.0GHz连续波或16.5GHz脉冲波暴露于人类淋巴细胞均未诱导染色体畸变频率出现统计学上的显著增加。在得出真正的阴性结论之前，16.5GHz脉冲波暴露需要进一步记录。
