Beliaeva N E, Garaeva L D, Timofeeva O A, Chulkova Iu Iu, Khokhlova L P
Department of Plant Physiology and Biotechnology of Kazan' State University.
Tsitologiia. 2002;44(5):485-90.
Effects of oryzalin (10 microM), an inhibitor of microtubule polymerization, on the activity of soluble and cell wall lectins were studied in 7 day-old seedlings of unhardened (23 degrees C) and cold acclimated (7 days at 2-3 degrees C) winter wheat (Triticum aestivum L.). Seedlings were grown in the presence of 25 microM and 1 mM Ca2+, 500 microM verapamil, 250 microM chlorpromazine or without modifiers of calcium status in the medium. Inhibitor of the microtubule polymerization inhibitor, likely as inhibitors of Ca(2+)-signal, decreased the activity of soluble lectins and increased that of cell wall lectins. Apparently, injury of microtubule phosphorylation results in a more considerable microtubule disorganization, than that observed after oryzalin effect. A low Ca2+ concentration (25 microM) depressed, while a high concentration (1 mM) prompted microtubule sensibility to oryzalin. Such an effect of high Ca2+ concentration may be related to destabilizative action of Ca(2+)-calmodulin in these conditions, because chlorpromazine decreased oryzalin-induced increase in the activity of cell wall lectins with 1 mM Ca2+. It is concluded that the activity of cell wall lectins depends on the microtubule status that is regulated by calcium signal.
在未驯化（23摄氏度）和冷驯化（2 - 3摄氏度，7天）的冬小麦（Triticum aestivum L.）7日龄幼苗中，研究了微管聚合抑制剂oryzalin（10微摩尔）对可溶性和细胞壁凝集素活性的影响。幼苗在含有25微摩尔和1毫摩尔Ca2 +、500微摩尔维拉帕米、250微摩尔氯丙嗪的条件下生长，或在培养基中不添加钙状态调节剂。微管聚合抑制剂oryzalin，可能作为Ca(2 +)信号抑制剂，降低了可溶性凝集素的活性并增加了细胞壁凝集素的活性。显然，微管磷酸化损伤导致的微管解聚比oryzalin作用后观察到的更显著。低Ca2 +浓度（25微摩尔）抑制，而高浓度（1毫摩尔）促使微管对oryzalin敏感。高Ca2 +浓度的这种作用可能与这些条件下Ca(2 +)-钙调蛋白的去稳定作用有关，因为氯丙嗪降低了1毫摩尔Ca2 +时oryzalin诱导的细胞壁凝集素活性增加。得出的结论是，细胞壁凝集素的活性取决于由钙信号调节的微管状态。
