Lavorante André F, Giné Maria Fernanda, Gervasio Ana Paula G, Miranda Carlos E S, Fiore Marli F, Bellato Cláudia M, Carrilho Emanuel
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário 303, 13416-000, Piracicaba, SP, Brazil.
Anal Sci. 2003 Dec;19(12):1611-6. doi: 10.2116/analsci.19.1611.
A home-made system hyphenating capillary electrophoresis with an inductively coupled plasma mass spectrometer (CE-ICP-MS) for cadmium speciation of protein-binding and free cadmium ions in solution is presented. The CE-ICP-MS interface consisted of an acrylic block with an internal volume ca. 20 microL in which a platinum electrode, a capillary column, and a connection to an ICP nebulizer were inserted. A make-up electrolyte solution containing 50 mmol L(-1) Tris-HCl buffer solution (pH 9.0) was continuously flowed through the interface to the ICP nebulizer. The separation of free Cd ions, Cd-cysteine, and Cd bounded to metallothionein (MT) isoforms from rabbit liver was carried out by capillary electrophoresis, and the analytes were detected by ICP-MS. The feasibility to isolate metallothionein compounds extracted from the cyanobacterium Synechococcus PCC7942 was demonstrated. The Cd binding proteins were induced in Synechococcus PCC7942 and further analyzed by CE ICP-MS.
本文介绍了一种自制的毛细管电泳与电感耦合等离子体质谱联用（CE-ICP-MS）系统，用于测定溶液中与蛋白质结合的镉和游离镉离子的形态。CE-ICP-MS接口由一个内部体积约为20微升的丙烯酸块组成，其中插入了一个铂电极、一根毛细管柱以及与ICP雾化器的连接管。含有50 mmol L(-1) Tris-HCl缓冲溶液（pH 9.0）的补充电解质溶液持续流经接口进入ICP雾化器。通过毛细管电泳分离兔肝中游离镉离子、镉-半胱氨酸以及与金属硫蛋白（MT）异构体结合的镉，并采用ICP-MS检测分析物。证明了分离从蓝藻聚球藻PCC7942中提取的金属硫蛋白化合物的可行性。在聚球藻PCC7942中诱导产生了镉结合蛋白，并通过CE ICP-MS进一步分析。
