金黄色葡萄球菌中毒性休克综合征毒素-1的纯化、结晶及初步X射线分析

Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus.

作者信息

Passalacqua E F, Brehm R D, Acharya K R, Tranter H S

机构信息

Department of Biochemistry, University of Bath, Avon, U.K.

出版信息

J Mol Biol. 1992 Dec 5;228(3):983-6. doi: 10.1016/0022-2836(92)90882-k.

DOI:10.1016/0022-2836(92)90882-k

PMID:1469731

Abstract

High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (> 99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C222(1), with unit cell dimensions a = 108.6 A, b = 177.6 A and c = 97.5 A, with three molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for three-dimensional X-ray structural analysis.

摘要

已采用一种新颖、简单的两步法，即利用染料配体色谱法，从金黄色葡萄球菌中纯化出了高产率的毒性休克综合征毒素-1（纯度>99%）。使用硫酸铵和聚乙二醇作为沉淀剂，通过气相扩散法获得了适合X射线衍射研究的晶体。它们属于正交晶系空间群C222(1)，晶胞参数a = 108.6 Å，b = 177.6 Å，c = 97.5 Å，每个不对称单元中有三个分子。这些晶体的衍射分辨率至少为2.5 Å，适用于三维X射线结构分析。