Spanová Alena, Horák Daniel, Soudková Eva, Rittich Bohuslav
Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):27-32. doi: 10.1016/j.jchromb.2003.09.010.
Magnetic hydrophilic non-porous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of several kinds of magnetite. It was found that some components used in the preparation of magnetic carriers interfered with polymerase chain reaction (PCR). Influence of non-magnetic and magnetic microspheres, including magnetite nanoparticles and various components used in their synthesis, on the PCR course was thus investigated. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interfering magnetic microspheres. The method enabled verification of the incorporation of magnetite nanoparticles in the particular methacrylate-based polymer microspheres and evaluation of suitability of their application in PCR. Preferably, electrostatically stabilized colloidal magnetite (ferrofluid) should be used in the design of new magnetic methacrylate-based microspheres by dispersion polymerization.
通过在几种磁铁矿存在下，使甲基丙烯酸2-羟乙酯（HEMA）与二甲基丙烯酸乙酯（EDMA）或甲基丙烯酸缩水甘油酯（GMA）进行分散（共）聚合，制备了磁性亲水性无孔P（HEMA-co-EDMA）、P（HEMA-co-GMA）和聚甲基丙烯酸缩水甘油酯（PGMA）微球。发现用于制备磁性载体的某些成分会干扰聚合酶链反应（PCR）。因此，研究了非磁性和磁性微球，包括磁铁矿纳米颗粒及其合成中使用的各种成分对PCR过程的影响。从长双歧杆菌细菌细胞中分离的DNA用于无干扰磁性微球的PCR评估。该方法能够验证磁铁矿纳米颗粒在特定甲基丙烯酸酯基聚合物微球中的掺入情况，并评估其在PCR中的应用适用性。在通过分散聚合设计新型磁性甲基丙烯酸酯基微球时，最好使用静电稳定的胶体磁铁矿（铁磁流体）。
