液相色谱法测定人血清中甲芬那酸的含量。
Liquid chromatography method for determination of mefenamic acid in human serum.
作者信息
Rouini Mohammad-Reza, Asadipour Ali, Ardakani Yalda Hoseinzadeh, Aghdasi Fakhredin
机构信息
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451, Iran.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):189-92. doi: 10.1016/j.jchromb.2003.09.063.
A simple, rapid and specific method for analysis of mefenamic acid (I) in serum by a sensitive high-performance liquid chromatography is described. Only 70 microl of serum and a little sample work-up is required. A simple procedure of extraction by dichloromethane followed by evaporation to dryness under gentle stream of nitrogen and dissolving the dried residue in mobile phase was used. The mefenamic acid peak was separated from endogenous peaks on a C(8) column by a mobile phase of acetonitrile-water (50:50, v/v, pH 3). Mefenamic acid and internal standard (IS) (diclofenac) were eluted at 7.4 and 5.4 min, respectively. The limit of quantitation of mefenamic acid in serum was 25 ng/ml at 280 nm. The method was linear over the range of 25-2000 ng/ml with r(2) of 0.998. Mean recovery for mefenamic acid was 110%.
描述了一种通过灵敏的高效液相色谱法分析血清中甲芬那酸（I）的简单、快速且特异的方法。仅需70微升血清及少量样品预处理。采用二氯甲烷简单萃取程序，随后在温和氮气流下蒸发至干，并将干燥残渣溶解于流动相中。在C(8)柱上，以乙腈 - 水（50:50，v/v，pH 3）的流动相将甲芬那酸峰与内源性峰分离。甲芬那酸和内标（IS）（双氯芬酸）分别在7.4分钟和5.4分钟洗脱。在280纳米处，血清中甲芬那酸的定量限为25纳克/毫升。该方法在25 - 2000纳克/毫升范围内呈线性，r(2)为0.998。甲芬那酸的平均回收率为110%。
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