Sokół Maria, Przybyszewski Waldemar M, Matlas Bozena
Department of Medical Physics, Institute of Oncology, ul. Wybrzeze Armii Krajowej, 15 Str., 44101 Gliwice, Poland.
Solid State Nucl Magn Reson. 2004 Jan;25(1-3):53-60. doi: 10.1016/j.ssnmr.2003.03.013.
The effect of irradiation on concentrations and relaxation behaviour of brain metabolites was studied by means of high-resolution 1H NMR in vitro. The studies were performed on rat brains irradiated with the doses of 20 Gy applied in fractions of 2 Gy. Standard procedures were used to obtain HClO4 extracts of rat brains. The 1H NMR studies of the extracts solutions in D2O were performed using a Varian Inova-300 NMR spectrometer. The integral intensities of the metabolite signals were found to change during the irradiation cycle and after it. These changes are accompanied by the variations in the T1 relaxation times. N-acetylaspartate, glycerophosphocholine, phosphocholine, choline, creatine and phosphocreatine, myoinositol and taurine were analysed as potential markers of irradiation injury.
通过高分辨率体外¹H NMR研究了辐照对脑代谢物浓度和弛豫行为的影响。研究在接受20 Gy剂量（分剂量为2 Gy）辐照的大鼠脑上进行。采用标准程序获取大鼠脑的高氯酸提取物。使用Varian Inova - 300 NMR光谱仪对D₂O中的提取物溶液进行¹H NMR研究。发现代谢物信号的积分强度在辐照周期期间及之后发生变化。这些变化伴随着T₁弛豫时间的改变。分析了N - 乙酰天门冬氨酸、甘油磷酸胆碱、磷酸胆碱、胆碱、肌酸和磷酸肌酸、肌醇和牛磺酸作为辐照损伤的潜在标志物。
