Lunam C A, Gentle M J
Department of Anatomy and Histology, School of Medicine, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia.
Neurosci Lett. 2004 Jan 9;354(2):87-90. doi: 10.1016/s0304-3940(03)00575-5.
Substance P immunoreactive (SP-IR) nerve fibres were identified using fluorescence immunohistochemistry and the length of the labelled individual nerve fibres was measured using confocal microscopy of whole mounts of the lateral wall of the ankle joint in 2-week-old domestic chicks. In the normal ankle there was an extensive network of SP-IR fibres in the synovial and subsynovial tissue of the joint capsule. Fours hours after injection of sodium urate into the joint space the joint was inflamed and there was a significant reduction in the labelled nerve fibres. These results demonstrate experimentally that painful gouty arthritis produces a neurogenic depletion of substance P from peripheral nerve fibres in the synovial and subsynovial tissue.
使用荧光免疫组织化学法鉴定P物质免疫反应性（SP-IR）神经纤维，并利用共聚焦显微镜对2周龄家鸡踝关节外侧壁整装片进行观察，测量标记的单个神经纤维的长度。在正常踝关节中，关节囊的滑膜和滑膜下组织中有广泛的SP-IR纤维网络。向关节腔内注射尿酸钠4小时后，关节发生炎症，标记的神经纤维显著减少。这些结果通过实验证明，疼痛性痛风性关节炎会导致滑膜和滑膜下组织中周围神经纤维的P物质发生神经源性耗竭。
