自身免疫小鼠大脑中的B细胞和T细胞。

B and T cells in the brains of autoimmune mice.

作者信息

Zameer Andleeb, Hoffman S A

机构信息

Department of Microbiology, Arizona State University, Tempe, AZ 85287-4501, USA.

出版信息

J Neuroimmunol. 2004 Jan;146(1-2):133-9. doi: 10.1016/j.jneuroim.2003.10.052.

DOI:10.1016/j.jneuroim.2003.10.052

PMID:14698855

Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disorder that can involve the central nervous system (CNS). Recently, we reported the presence of autoantibodies bound to the brain tissue of murine models of lupus; MRL/lpr and BXSB. We postulated that the source of these autoantibodies was in part due to in situ production, caused by the entry of B and T cells. Frozen brain sections of MRL/lpr and BXSB at 1 and 4 months of age were stained for CD3 (T cells) and CD19 (B cells) markers using an immunofluorescent antibody binding assay. Confocal fluorescence microscopy showed both CD3(+) and CD19(+) cells at 4 months of age only in MRL/lpr mice. There were no lymphocytes seen in the other autoimmune model, BXSB. Results suggest a difference in the mechanisms by which autoantibodies access the brain in these two autoimmune models of lupus.

摘要

系统性红斑狼疮（SLE）是一种可累及中枢神经系统（CNS）的自身免疫性疾病。最近，我们报道了在狼疮小鼠模型（MRL/lpr和BXSB）的脑组织中存在自身抗体。我们推测这些自身抗体的来源部分是由于B细胞和T细胞进入导致的原位产生。使用免疫荧光抗体结合试验对1月龄和4月龄的MRL/lpr和BXSB小鼠的冷冻脑切片进行CD3（T细胞）和CD19（B细胞）标记染色。共聚焦荧光显微镜显示，仅在4月龄的MRL/lpr小鼠中存在CD3(+)和CD19(+)细胞。在另一种自身免疫模型BXSB中未观察到淋巴细胞。结果表明，在这两种狼疮自身免疫模型中，自身抗体进入大脑的机制存在差异。