I型干扰素对单核细胞亚群及其向树突状细胞分化的影响。对接受β-干扰素治疗的多发性硬化症患者的一项体内和体外研究。

Impact of type-I-interferon on monocyte subsets and their differentiation to dendritic cells. An in vivo and ex vivo study in multiple sclerosis patients treated with interferon-beta.

作者信息

Then Bergh F, Dayyani Farshid, Ziegler-Heitbrock Loems

机构信息

Institute of Immunology, Ludwig-Maximilians-Universität, Munich, Germany.

出版信息

J Neuroimmunol. 2004 Jan;146(1-2):176-88. doi: 10.1016/j.jneuroim.2003.10.037.

DOI:10.1016/j.jneuroim.2003.10.037

PMID:14698861

Abstract

In addition to CD14++ "classical" monocytes, human peripheral blood contains CD14+CD16+ "pro-inflammatory" monocytes, which may be influenced by IFNb treatment. By fluorescence activated cell sorting (FACS) analysis, 94 multiple sclerosis (MS) patients revealed normal absolute and relative numbers of both monocyte populations (71 untreated, 23 IFNb-treated). In IFNb-treated patients, CD14+CD16+ monocytes consistently expressed higher CD14, confirmed in 16 patients analyzed longitudinally. Ex vivo, CD1a+CD14+ dendritic cells (DC) were efficiently differentiated from peripheral blood cells from controls and untreated patients, but at considerably reduced efficiency in IFNb-treated patients. Addition of IFNb to the medium further reduced the induction of CD1a+CD14+ cells.IFNb induces a novel immunophenotypic shift in pro-inflammatory monocytes, which appears to be related to reduced formation of dendritic cell precursors.

摘要

除了CD14++“经典”单核细胞外，人类外周血还含有CD14+CD16+“促炎”单核细胞，其可能受干扰素β（IFNβ）治疗的影响。通过荧光激活细胞分选（FACS）分析，94例多发性硬化症（MS）患者的两种单核细胞群的绝对数量和相对数量均正常（71例未治疗，23例接受IFNβ治疗）。在接受IFNβ治疗的患者中，CD14+CD16+单核细胞持续高表达CD14，这在16例纵向分析的患者中得到证实。在体外，CD1a+CD14+树突状细胞（DC）能有效地从对照和未治疗患者的外周血细胞中分化出来，但在接受IFNβ治疗的患者中分化效率显著降低。向培养基中添加IFNβ会进一步减少CD1a+CD14+细胞的诱导。IFNβ在促炎单核细胞中诱导了一种新的免疫表型转变，这似乎与树突状细胞前体形成减少有关。