Siedlar M, Frankenberger M, Ziegler-Heitbrock L H, Belge K U
Institute of Immunology, University of München, Germany.
Immunobiology. 2000 May;202(1):11-7. doi: 10.1016/s0171-2985(00)80047-9.
Recently, a population of M-DC8-positive leukocytes has been described as a new subpopulation of human dendritic cells (DC). In view of the expression of the CD16 antigen on these cells, as well as the finding that DC can arise from blood monocytes, we hypothesized that the expression of M-DC8 is mainly associated with the CD14+ CD16+ phenotype of blood monocytes. Immunofluorescence analysis of whole blood showed that the percentage of M-DC8+ cells is about three times lower than the percentage of CD14+ CD16+ monocytes among all leukocytes (0.32% versus 1.10%). Further, in addition to the expression of CD16, these M-DC8+ cells were also positive for CD14 at low density. Multicolor flow cytometric analysis of whole blood revealed that more than one third of the CD14+ CD16+ monocyte population expressed the M-DC8 antigen (42.3%), and almost all M-DC8+ cells were CD14-CD16-double-positive (87.5%). Finally, the M-DC8 antigen was also expressed on alveolar macrophages from healthy individuals, i.e., cells that are phenotypically and functionally related to the CD14+ CD16+ monocytes. Taken together, the data presented here clearly demonstrate that the M-DC8+ leukocytes are a subpopulation of the CD14+ CD16+ monocytes and may represent DC
最近，一群M-DC8阳性白细胞被描述为人类树突状细胞（DC）的一个新亚群。鉴于这些细胞上CD16抗原的表达，以及DC可源自血液单核细胞这一发现，我们推测M-DC8的表达主要与血液单核细胞的CD14+CD16+表型相关。全血免疫荧光分析显示，在所有白细胞中，M-DC8+细胞的百分比约比CD14+CD16+单核细胞的百分比低三倍（分别为0.32%和1.10%）。此外，除了CD16的表达外，这些M-DC8+细胞低密度时CD14也呈阳性。全血多色流式细胞术分析显示，超过三分之一的CD14+CD16+单核细胞群体表达M-DC8抗原（42.3%），几乎所有M-DC8+细胞都是CD14-CD16双阳性（87.5%）。最后，健康个体的肺泡巨噬细胞上也表达M-DC8抗原，即与CD14+CD16+单核细胞在表型和功能上相关的细胞。综上所述，此处呈现的数据清楚地表明，M-DC8+白细胞是CD14+CD16+单核细胞的一个亚群，可能代表DC
