Karpova E A, Kubareva E A, Bur'ianov Ia I, Gromova E S
Mol Biol (Mosk). 1992 Sep-Oct;26(5):993-8.
Binding of EcoRII restriction endonuclease to synthetic oligodeoxyribonucleotide substrates of 11-30 base pairs long was investigated by polyacrylamide gel electrophoresis under nondenaturing conditions in the absence of Mg2+ ions. Irrespective of the length of a substrate, two types of specific DNA-protein complexes were shown to be formed. Their mobility in gel was close to that of the monomer (45 kDa) and dimer (90 kDa) of marker protein, ovalbumin. The ratio of these complexes in solution depended on that of the molar concentrations of EcoRII restriction endonuclease and DNA duplexes. The possible structure of the complexes is discussed.
在无Mg2+离子的非变性条件下，通过聚丙烯酰胺凝胶电泳研究了EcoRII限制性内切酶与11 - 30个碱基对长的合成寡脱氧核糖核苷酸底物的结合。无论底物长度如何，都显示形成了两种类型的特异性DNA - 蛋白质复合物。它们在凝胶中的迁移率接近标记蛋白卵清蛋白的单体（45 kDa）和二聚体（90 kDa）。溶液中这些复合物的比例取决于EcoRII限制性内切酶和DNA双链体的摩尔浓度比例。讨论了复合物可能的结构。
