[从人胎盘中分离DNA依赖性DNA聚合酶ε的方法及其性质]
[A method of isolation and properties of DNA-dependent DNA-polymerase epsilon from human placenta].
作者信息
Mozzherin D Iu, Atrazhev A M, Kukhanova M K
出版信息
Mol Biol (Mosk). 1992 Sep-Oct;26(5):999-1010.
PMID:1470182
Abstract
DNA polymerase epsilon was purified to near homogeneity from human placenta. The enzyme has one subunit (170 kDa, sedimentation coefficient 8.2S), intrinsic 3'-5'-exonuclease activity, it is independent on PCNA and high processivity on poly(dA)-oligo(dT) template-primer without PCNA. It was shown, that the enzyme incorporates 3'-amino-2',3'-dideoxythymidine 5'-triphosphate in DNA, after that synthesis is stopped. Simultaneously DNA polymerase alpha was purified.
摘要
从人胎盘中将DNA聚合酶ε纯化至接近均一状态。该酶有一个亚基（170 kDa，沉降系数8.2S），具有内在的3'-5'-外切核酸酶活性，不依赖增殖细胞核抗原（PCNA），在没有PCNA的情况下对聚（dA）-寡聚（dT）模板引物具有高持续合成能力。结果表明，该酶将3'-氨基-2'，3'-双脱氧胸苷5'-三磷酸掺入DNA后，合成即停止。同时，对DNA聚合酶α进行了纯化。
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