[免疫组织化学检测hMLH1和hMSH2及微卫星不稳定性在鉴定遗传性非息肉病性结直肠癌家系中的作用]
[The role of the immunohistochemistry for hMLH1 and hMSH2 with detection of microsatellite instability to identify the kindreds with hereditary nonpolyposis colorectal cancer].
作者信息
Jin Hei-ying, Cui Long, Meng Rong-gui, Liu Fei, Yan Yu-di, Ding Yi-jiang, Yao Hang, Fu Chuan-gang, Yu De-hong
机构信息
Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2003 Nov;41(11):809-11.
OBJECTIVE
To investigate the specificity and sensitivity of the immunohistochemistry for hMLH1 and hMSH2 with detection of microsatellite instability (MSI) to identify the kindreds with hereditary nonpolyposis colorectal cancer and to analyse its value in clinical practice.
METHOD
Specimens of 16 cases with HNPCC and 16 cases with sporadic colorectal cancer were detected by immunostaining with hMLH1 and hMSH2 and MSI was also detected.
RESULTS
The specificity and sensitivity of the immunohistochemistry for hMLH1 and hMSH2 were 91.7% and 87.5% respectively. The specificity and sensitivity of MSI were 100% and 75.0%. By combining two methods, the specificity and sensitivity were 91.7% and 93.8% respectively.
CONCLUSIONS
By combination of the immunohistochemistry for hMLH1 and hMSH2 and detection of MSI to identify the kindreds with HNPCC, the specificity and sensitivity are improved which is better than to use either of them alone. And it is very easy and cheap that it can be used in clinics.
目的
探讨hMLH1和hMSH2免疫组化检测微卫星不稳定性（MSI）以识别遗传性非息肉病性结直肠癌家系的特异性和敏感性，并分析其在临床实践中的价值。
方法
采用hMLH1和hMSH2免疫染色法检测16例遗传性非息肉病性结直肠癌（HNPCC）患者和16例散发性结直肠癌患者的标本，并检测MSI。
结果
hMLH1和hMSH2免疫组化的特异性和敏感性分别为91.7%和87.5%。MSI的特异性和敏感性分别为100%和75.0%。两种方法联合使用时，特异性和敏感性分别为91.7%和93.8%。
结论
联合应用hMLH1和hMSH2免疫组化及MSI检测来识别遗传性非息肉病性结直肠癌家系，可提高特异性和敏感性，比单独使用任何一种方法都更好。而且该方法操作简便、成本低廉，可应用于临床。
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