Noctor Stephen C, Martínez-Cerdeño Verónica, Ivic Lidija, Kriegstein Arnold R
Department of Neurology, Columbia University College of Physicians & Surgeons, 630 W. 168th Street, New York, New York 10032, USA.
Nat Neurosci. 2004 Feb;7(2):136-44. doi: 10.1038/nn1172. Epub 2004 Jan 4.
Precise patterns of cell division and migration are crucial to transform the neuroepithelium of the embryonic forebrain into the adult cerebral cortex. Using time-lapse imaging of clonal cells in rat cortex over several generations, we show here that neurons are generated in two proliferative zones by distinct patterns of division. Neurons arise directly from radial glial cells in the ventricular zone (VZ) and indirectly from intermediate progenitor cells in the subventricular zone (SVZ). Furthermore, newborn neurons do not migrate directly to the cortex; instead, most exhibit four distinct phases of migration, including a phase of retrograde movement toward the ventricle before migration to the cortical plate. These findings provide a comprehensive and new view of the dynamics of cortical neurogenesis and migration.
精确的细胞分裂和迁移模式对于将胚胎前脑的神经上皮转化为成人大脑皮层至关重要。通过对大鼠皮层中几代克隆细胞进行延时成像，我们在此表明，神经元通过不同的分裂模式在两个增殖区产生。神经元直接由脑室区（VZ）的放射状胶质细胞产生，间接由室下区（SVZ）的中间祖细胞产生。此外，新生神经元不会直接迁移到皮层；相反，大多数神经元表现出四个不同的迁移阶段，包括在迁移到皮层板之前向脑室逆行运动的阶段。这些发现为皮层神经发生和迁移的动力学提供了一个全面的新视角。
