Konopacka M, Wideł M, Grzybowska E, Suwiński J
Laboratory of Radiobiology, M. Skłodowska-Curie Memorial Institute, Gliwice, Poland.
Pol J Pharmacol Pharm. 1992 May-Jun;44(3):299-306.
The hypoxic radiosensitizer 1-(2-hydroxy-3-methoxypropyl)-2-chloro-4-nitroimidazole [P40] was investigated for its mutagenic activity in bacterial Ames test as well as for genotoxic activity in micronucleus assay in vivo. This nitroimidazole showed the weak mutagenicity towards TA100 strain (base pair substitution) and towards TA98 strain (frameshift) only in the highest concentration. P40 induced also a significant increase in the frequency of micronucleated polychromatic erythrocytes (PCEs) at the doses of 0.6 mg/g and 1.2 mg/g. The maximum time response was at 48 h. The decrease of percentage of PCEs suggested the possible cytotoxicity on bone marrow cells after treatment with P40. Positive results in this battery short-term tests provide evidence of clastogenic activity of P40.
对缺氧放射增敏剂1-(2-羟基-3-甲氧基丙基)-2-氯-4-硝基咪唑[P40]进行了细菌艾姆斯试验中的致突变活性以及体内微核试验中的遗传毒性活性研究。这种硝基咪唑仅在最高浓度下对TA100菌株（碱基对置换）和TA98菌株（移码）表现出微弱的致突变性。P40在剂量为0.6 mg/g和1.2 mg/g时也诱导微核多染红细胞（PCEs）频率显著增加。最大时间反应在48小时。PCEs百分比的降低表明用P40处理后对骨髓细胞可能具有细胞毒性。该系列短期试验的阳性结果提供了P40具有断裂剂活性的证据。
