Sun X B, Matsumoto T, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Planta Med. 1992 Oct;58(5):445-8. doi: 10.1055/s-2006-961510.
Water-soluble and alkaline-soluble crude polysaccharides which were separated from the roots or leaves of Panax ginseng C. A. Meyer, were compared for their anti-ulcer activity. Of these four polysaccharide fractions, the water-soluble crude polysaccharide fraction (GL-2) from the leaves and the alkaline-soluble crude polysaccharide fraction (GRA-2) from the roots prevented HCl/ethanol-induced ulcerogenesis in mice potently. The most potent fraction, GL-2, was further fractionated into four polysaccharide fractions by precipitation with cethyltrimethylammonium bromide, and the weakly acidic polysaccharide fraction, GL-4, showed the most potent inhibition of gastric lesion formation. The activity of GL-4 decreased after treatment with periodate or digestion with endo-polygalacturonase, indicating that the carbohydrate moiety may contribute to the expression of the activity. GL-4 was further purified by anion-exchange chromatography and gel filtration, and the most active purified polysaccharide, GL-4IIb1III was obtained. GL-4IIb1III (average relative molecular mass, 16,000 d) had the nature of a pectic polysaccharide, and was composed mainly of galactose and galacturonic acid with small proportions of rhamnose, arabinose, mannose, glucose, and glucuronic acid. GL-4IIIb1III prevented HCl/ethanol-induced ulcerogenesis in mice dose dependently.
对从人参（Panax ginseng C. A. Meyer）根或叶中分离得到的水溶性粗多糖和碱溶性粗多糖的抗溃疡活性进行了比较。在这四种多糖组分中，叶中的水溶性粗多糖组分（GL - 2）和根中的碱溶性粗多糖组分（GRA - 2）对小鼠盐酸/乙醇诱导的溃疡形成有很强的预防作用。最有效的组分GL - 2通过十六烷基三甲基溴化铵沉淀进一步分离成四个多糖组分，其中弱酸性多糖组分GL - 4对胃损伤形成的抑制作用最强。用高碘酸盐处理或用内切聚半乳糖醛酸酶消化后，GL - 4的活性降低，表明碳水化合物部分可能有助于该活性的表达。GL - 4通过阴离子交换色谱和凝胶过滤进一步纯化，得到了活性最强的纯化多糖GL - 4IIb1III。GL - 4IIb1III（平均相对分子质量为16,000 d）具有果胶多糖的性质，主要由半乳糖和半乳糖醛酸组成，含有少量鼠李糖、阿拉伯糖、甘露糖、葡萄糖和葡萄糖醛酸。GL - 4IIIb1III对小鼠盐酸/乙醇诱导的溃疡形成具有剂量依赖性的预防作用。
