Brustein Edna, Saint-Amant Louis, Buss Robert R, Chong Mabel, McDearmid Jonathan R, Drapeau Pierre
McGill Centre for Research in Neuroscience, McGill University, 1650 Cedar Avenue, Montreal, Quebec, Canada H3G 1A4.
J Physiol Paris. 2003 Jan;97(1):77-86. doi: 10.1016/j.jphysparis.2003.10.009.
This review summarizes recent data from our lab concerning the development of motor activities in the developing zebrafish. The zebrafish is a leading model for studies of vertebrate development because one can obtain a large number of transparent, externally and rapidly developing embryos with motor behaviors that are easy to assess (e.g. for mutagenic screens). The emergence of embryonic motility was studied behaviorally and at the cellular level. The embryonic behaviors appear sequentially and include an early, transient period of spontaneous, alternating tail coilings, followed by responses to touch, and swimming. Patch clamp recording in vivo revealed that an electrically coupled network of a subset of spinal neurons generates spontaneous tail coiling, whereas a chemical (glutamatergic and glycinergic) synaptic drive underlies touch responses and swimming and requires input from the hindbrain. Swimming becomes sustained in larvae once serotonergic neuromodulatory effects are integrated. We end with a brief overview of the genetic tools available for the study of the molecular determinants implicated in locomotor network development in the zebrafish. Combining genetic, behavioral and cellular experimental approaches will advance our understanding of the general principles of locomotor network assembly and function.
本综述总结了我们实验室最近关于斑马鱼幼体运动活动发育的研究数据。斑马鱼是脊椎动物发育研究的主要模型，因为可以获得大量透明、外部发育迅速且运动行为易于评估的胚胎（例如用于诱变筛选）。从行为和细胞水平研究了胚胎运动的出现。胚胎行为依次出现，包括早期短暂的自发交替尾卷曲期，随后是对触摸的反应和游泳。体内膜片钳记录显示，脊髓神经元子集的电耦合网络产生自发尾卷曲，而化学（谷氨酸能和甘氨酸能）突触驱动是触摸反应和游泳的基础，并且需要来自后脑的输入。一旦整合了血清素能神经调节作用，幼体中的游泳就会持续。我们最后简要概述了可用于研究斑马鱼运动网络发育中涉及的分子决定因素的遗传工具。结合遗传、行为和细胞实验方法将推进我们对运动网络组装和功能一般原理的理解。
