Kanna P Suresh, Mahendrakumar C B, Chatterjee M, Hemalatha P, Datta Subroto, Chakraborty Prabir
Division of Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
J Biochem Mol Toxicol. 2003;17(6):357-65. doi: 10.1002/jbt.10099.
Vanadium (V) has recently been found to possess potent anti-neoplastic activity in rat colon carcinogenesis. In the present study attempts have been made to investigate the expression of the number and area of aberrant crypt foci positive for placental glutathione S-transferase (GST-P) during 1,2-dimethyl hydrazine (DMH)-induced rat colon carcinogenesis. Male Sprague Dawley rats were randomly divided into four groups. Rats in group A were designed as normal controls. Group B animals received DMH once a week (20 mg/kg body wt.) intraperitoneally for 16 weeks. Group C rats received the same treatment of DMH as in group B, along with 0.5-ppm vanadium as ammonium monovanadate ad libitum in drinking water throughout the experiment. Vanadium alone was given to Group D rats without any DMH injection. The expression of the number and the area of aberrant crypt foci (ACF) positive for GST-P was maximum in DMH-treated group. Vanadium-treated rats significantly reduced (P < 0.001) the expression of GST-P positive ACF cells (by 71.13%) for the entire period of the study. Moreover the histopathological examination also showed that vanadium action could minimize the aberrant crypt foci (P < 0.001). Furthermore, vanadium supplementation also elevated SOD activities in both liver and colon (P < 0.01, P < 0.02 and P < 0.01, P < 0.02 respectively) when compared to their carcinogen counterparts. Our results confirm that vanadium is particularly effective in limiting the action of the carcinogen, thereby establishing its anticarcinogenicity in chemically induced rat colon carcinogenesis.
最近发现钒（V）在大鼠结肠癌发生过程中具有强大的抗肿瘤活性。在本研究中，已尝试调查在1,2 - 二甲基肼（DMH）诱导的大鼠结肠癌发生过程中，胎盘型谷胱甘肽S - 转移酶（GST - P）阳性的异常隐窝灶数量和面积的表达情况。雄性Sprague Dawley大鼠被随机分为四组。A组大鼠被设计为正常对照组。B组动物每周腹腔注射一次DMH（20毫克/千克体重），共注射16周。C组大鼠接受与B组相同的DMH处理，并且在整个实验过程中，饮用水中随意添加0.5 ppm的钒，以偏钒酸铵形式提供。D组大鼠只给予钒，不注射任何DMH。在DMH处理组中，GST - P阳性的异常隐窝灶（ACF）数量和面积的表达最高。在整个研究期间，钒处理的大鼠显著降低了（P < 0.001）GST - P阳性ACF细胞的表达（降低了71.13%）。此外，组织病理学检查还表明，钒的作用可以使异常隐窝灶数量最小化（P < 0.001）。此外，与致癌物处理组相比，补充钒还提高了肝脏和结肠中的超氧化物歧化酶（SOD）活性（分别为P < 0.01、P < 0.02和P < 0.01、P < 0.02）。我们的结果证实，钒在限制致癌物的作用方面特别有效，从而在化学诱导的大鼠结肠癌发生过程中确立了其抗癌性。
