Rolland C, Bailleux B, Biausque S, Fortin L
Laboratoire polyvalent de l'hôpital, Centre Hospitalier de Seclin, Allée des Marronniers, 59471 Seclin.
Ann Biol Clin (Paris). 2003 Nov-Dec;61(6):705-8.
Granada medium (GM) was evaluated for the detection of group B streptococci (GBS) in vaginal swabs compared with the standard culture on selective blood agar (BA) and classical identification methods. From May to November 2002, samples from 325 pregnant women (34 to 37 weeks of gestation) were processed and 44 of these women (13.5%) carried GBS. Comparatively, GM was found more sensitive than the selective BA (95% versus 91%) in GBS recovery. The characteristic red-orange colonies produced by GBS are so specific that further identification is unnecessary. The technique is simple and results are available after overnight incubation, improving the time to reporting a GBS-positive result by at least 24 h. The inconvenience of anaerobic incubation of GM plates can be avoided when a cover slide is placed upon the inoculum because the same pigmentation is obtained under these aerobic conditions. This study confirms that the routine use of GM appears to be an accurate, easy and highly sensitive method of identification of GBS in pregnant women.
与在选择性血琼脂（BA）上进行标准培养及经典鉴定方法相比，对格拉纳达培养基（GM）检测阴道拭子中B族链球菌（GBS）的情况进行了评估。2002年5月至11月，对325名孕妇（妊娠34至37周）的样本进行了处理，其中44名女性（13.5%）携带GBS。相比之下，发现GM在GBS回收方面比选择性BA更敏感（95%对91%）。GBS产生的特征性红橙色菌落非常特异，无需进一步鉴定。该技术简单，过夜培养后即可获得结果，将GBS阳性结果的报告时间至少缩短了24小时。当在接种物上放置盖玻片时，可避免GM平板厌氧培养的不便，因为在这些有氧条件下可获得相同的色素沉着。本研究证实，常规使用GM似乎是一种准确、简便且高度灵敏的鉴定孕妇GBS的方法。
