从细菌培养上清液中沉淀蛋白质的简单可靠方法。
Simple and reliable method to precipitate proteins from bacterial culture supernatant.
作者信息
Caldwell Randolph B, Lattemann Claus T
机构信息
CREATOGEN AG, D-86156 Augsburg, Germany.
出版信息
Appl Environ Microbiol. 2004 Jan;70(1):610-2. doi: 10.1128/AEM.70.1.610-612.2004.
Abstract
A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.
摘要
基于邻苯三酚红-钼酸盐-甲醇（PRMM）方案，开发了一种从细菌培养上清液中沉淀蛋白质的简单可靠方法，并将其应用于分析细菌III型分泌系统分泌的蛋白质。已证明基于PRMM的沉淀比传统方案更高效、更可靠。
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