Kaderlik R K, Lin D X, Lang N P, Kadlubar F F
National Center for Toxicological Research, Jefferson, AR 72079.
Toxicol Lett. 1992 Dec;64-65 Spec No:469-75. doi: 10.1016/0378-4274(92)90221-5.
In molecular epidemiological studies, the measurement of carcinogen-DNA adducts in human tissues can provide direct evidence of current exposure to chemical carcinogens. Moreover, data on steady state DNA adduct levels and the rate of cell proliferation can be related not only to carcinogen-target tissue dosimetry but may also be useful in assessment of human cancer risk. Thus far, laboratory methods for adduct detection have primarily utilized 32P-postlabelling, immunoassays, and mass spectrometry. However, accurate quantitation of DNA adducts requires knowledge of the structural identity and chemical properties of carcinogen-base adducts, the availability of synthetic standards for recovery determinations, and the development of complementary methods to corroborate analytical findings.
在分子流行病学研究中，测量人体组织中的致癌物 - DNA加合物可以提供当前接触化学致癌物的直接证据。此外，关于稳态DNA加合物水平和细胞增殖速率的数据不仅可以与致癌物 - 靶组织剂量测定相关，还可能有助于评估人类癌症风险。迄今为止，加合物检测的实验室方法主要采用32P后标记法、免疫测定法和质谱分析法。然而，DNA加合物的准确定量需要了解致癌物 - 碱基加合物的结构特性和化学性质、用于回收率测定的合成标准品的可用性，以及开发互补方法以证实分析结果。
